Platelet-rich plasma: Growth factors and pro- and anti-inflammatory properties
Platelet-rich plasma: Growth factors and pro- and anti-inflammatory properties
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DOI：
10.1902/jop.2007.060302
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发表时间：
2007-04-01
影响因子：
4.3
通讯作者：
Van Dyke, Thomas E.
中科院分区：
文献类型：
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作者：
El-Sharkawy, Hesham;Kantarci, Alpdogan;Van Dyke, Thomas E.
背景：富含血小板的血浆（PRP）可能通过浓缩生长因子的作用促进骨再生。然而，目前尚不清楚 PRP 如何影响炎症反应。本研究的目的是分析PRP中的生长因子，研究PRP对单核细胞细胞因子释放和脂氧素A（4）（LXA（4））生成的影响。方法：PRP由健康供体制备。通过酶联免疫吸附测定评估血小板源性生长因子（PDGF）-AB、PDGF-BB、转化生长因子-β1、胰岛素样生长因子-I、碱性成纤维细胞生长因子（FGF-b）、表皮生长因子（EGF）、血管内皮生长因子、白介素-12（p40/70）以及激活调节、正常T细胞表达和分泌（RANTES）水平基于珠的多重分析。分离外周血单核细胞并在有或没有PRP的情况下培养。分析了细胞因子、趋化因子和 LXA(4) 水平以及单核细胞趋化性迁移。结果：与全血 (WB) 和贫血小板血浆相比，PRP 中的生长因子显着增加。 PRP 显着抑制单核细胞趋化蛋白-1 (MCP-1)，而单核细胞培养物中 RANTES 显着增加。与 WB 相比，PRP 中的 LXA(4) 水平显着较高。 PRP 以剂量依赖性方式刺激单核细胞趋化性，而 RANTES 在一定程度上负责 PRP 介导的单核细胞迁移。结论：PRP 是生长因子的丰富来源，并促进单核细胞介导的促炎细胞因子/趋化因子释放的显着变化。 PRP 中 LXA(4) 增加，表明 PRP 可能抑制细胞因子释放、限制炎症，从而促进组织再生。
Background: Platelet-rich plasma (PRP) promotes regeneration of bone, presumably through the action of concentrated growth factors. However, it is not clear how PRP affects the inflammatory response. The purpose of this study was to analyze the growth factors in PRP and to study the effects of PRP on monocyte cytokine release and lipoxin A(4) (LXA(4)) generation.Methods: PRP was prepared from healthy donors. Platlet-derived growth factor (PDGF)-AB, PDGF-BB, transforming growth factor-beta 1, insulin-like growth factor-I, fibroblast growth factor-basic (FGF-b), epidermal growth factor (EGF), vascular endothelial growth factor, interleukin-12 (p40/70), and regulated on activation, normal T-cell expressed and secreted (RANTES) levels were evaluated by enzyme-linked immunosorbent assay and bead-based multiplexing. Peripheral blood monocytes were isolated and cultured with or without PRP. Cytokine, chemokine, and LXA(4) levels as well as monocyte chemotactic migration were analyzed.Results: Growth factors were increased significantly in PRP compared to whole blood (WB) and platelet-poor plasma. Monocyte chemotactic protein-1 (MCP-1) was suppressed significantly by PRP, whereas RANTES was increased significantly in monocyte cultures. LXA(4) levels were significantly higher in PRP compared to WB. PRP stimulated monocyte chemotaxis in a dose-dependent fashion, whereas RANTES, in part, was responsible for PRP-mediated monocyte migration.Conclusion: PRP is a rich source of growth factors and promoted significant changes in monocyte-mediated proinflammatory cytokine/chemokine release. LXA(4) was increased in PRP, suggesting that PRP may suppress cytokine release, limit inflammation, and, thereby, promote tissue regeneration.