Crytosporidium spp. from small mammals in the New York City watershed
Crytosporidium spp. from small mammals in the New York City watershed
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DOI：
10.7589/0090-3558-43.4.586
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发表时间：
2007-10-01
影响因子：
1.3
通讯作者：
Mohammed, Hussni O.
中科院分区：
文献类型：
--
作者：
Ziegler, Peter E.;Wade, Susan E.;Mohammed, Hussni O.
本研究的目的是评估野生动物通过山雀隐孢子虫基因型污染水源而在流域环境退化中所起的潜在作用。采用针对S-Small Subunit (SSU) rRNA位点的聚合酶链反应方法，对从纽约市（NYC）流域野生动物中分离的隐孢子虫进行了基因型检测。从纽约流域捕获的12种野生动物宿主物种中提取的77个DNA样本进行了扩增和测序。还收集和分析了与这些基因型延续相关的危险因素数据。尽管许多基因型似乎是宿主特异性的，但检查的样本中有38%被鉴定为小隐孢子虫，这表明存在人畜共患隐孢子虫。成年动物更容易脱落隐孢子虫，秋冬季节捕获的动物比春夏季节捕获的动物更容易感染小隐孢子虫。
The objective of this study was to assess the potential role that wildlife plays in environmental degradation of watersheds through the contamination of the water supply with zoonotic genotypes of Cryptosporidium tit. Cryptosporidium isolates recovered from wildlife in the New York City (NYC) watershed were examined to determine genotype using a polymerase chain reaction protocol targeting the I S-Small Subunit (SSU) rRNA locus. Seventy-seven DNA samples recovered from 12 wildlife host species captured in the NYC watershed were amplified and sequenced. Data on risk factors associated with the perpetuation of these genotypes also were collected and analyzed. Although many genotypes appeared to be host-specific, 38% of the samples examined were identified as Cryptosporidium parvum, indicating the presence of zoonotic Cryptosporidium. Adult animals were more likely to shed the zoonotic strains of Cryptosporidium spp. Animals captured in the fall and winter were more likely to be infected with C. parvum than those captured in spring and summer.