Time-course changes in the expression of Na, K-ATPase and the morphometry of mitochondrion-rich cells in gills of euryhaline tilapia (Oreochromis mossambicus) during freshwater acclimation.
Time-course changes in the expression of Na, K-ATPase and the morphometry of mitochondrion-rich cells in gills of euryhaline tilapia (Oreochromis mossambicus) during freshwater acclimation.
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淡水驯化过程中广盐罗非鱼（Oreochromis mossambicus）鳃中Na、K-ATP酶表达和富含线粒体细胞形态的时程变化。
DOI：
10.1002/jez.a.20007
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Hwang,Pung-Pung
中科院分区：
文献类型：
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作者：
Lin,Chia-Hao;Huang,Chao-Lu;Yang,Chi-Hwa;Lee,Tsung-Han;Hwang,Pung-Pung
研究人员在从海水 (SW) 转移到淡水 (FW) 后 96 小时的时间过程中研究了罗非鱼鳃中 Na、K-ATP 酶 (NKA) 表达和富含线粒体 (MR) 细胞形态的变化。从 3 小时起，血浆渗透压和 Na+、Cl− 浓度出现短暂下降。 Gills 对 FW 转移的反应是早在转移后 3 小时就降低 NKA 活性。这种反应之后，NKA 同工型 α1-mRNA 丰度显着下降，这是在转移后 6 小时通过实时 PCR 检测到的。接下来，从转移后 6 小时到 24 小时观察到 α1-蛋白量减少。此外，在 FW 转移过程中，观察到鳃 MR 细胞亚型的数量和大小发生变化，尽管所有 MR 细胞亚型的密度没有发现显着差异。这些修饰早在 3 小时就被发现，在 6 小时就很明显（表现出 3 种 MR 细胞亚型），并且大部分在转移后 24 小时完成。这种快速反应（3小时内），如鳃NKA表达的同时变化和MR细胞亚型的修饰，被认为可以提高罗非鱼从高渗SW到低渗FW的适应能力。过期。动物园。 301A:85–96, 2004。© 2004 Wiley-Liss, Inc.
Changes in expression of Na, K‐ATPase (NKA) and morphometry of mitochondrion‐rich (MR) cells in gills of tilapia were investigated on a 96–hr time course following transfer from seawater (SW) to fresh water (FW). A transient decline in plasma osmolality and Na+, Cl−concentrations occurred from 3 hrs onward. Gills responded to FW transfer by decreasing NKA activity as early as 3 hrs from transfer. This response was followed by a significant decrease in the NKA isoform α1–mRNA abundance, which was detected by real‐time PCR at 6 hrs post transfer. Next, a decrease of α1–protein amounts were observed from 6 hrs until 24 hrs post transfer. Additionally, during the time course of FW transfer, modifications in number and size of subtypes of gill MR cells were observed although no significant difference was found in densities of all subtypes of MR cells. These modifications were found as early as 3 hrs, evident at 6 hrs (exhibition of 3 subtypes of MR cells), and mostly completed by 24 hrs post transfer. Such rapid responses (in 3 hrs) as concurrent changes in branchial NKA expression and modifications of MR cell subtypes are thought to improve the osmoregulatory capacity of tilapia in acclimation from hypertonic SW to hypotonic FW.J. Exp. Zool. 301A:85–96, 2004. © 2004 Wiley‐Liss, Inc.