Establishment of a bilateral femoral large segmental bone defect mouse model potentially applicable to basic research in bone tissue engineering
Establishment of a bilateral femoral large segmental bone defect mouse model potentially applicable to basic research in bone tissue engineering
复制标题
双侧股骨大节段骨缺损小鼠模型的建立，有望应用于骨组织工程基础研究
DOI：
10.1016/j.jss.2014.05.037
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发表时间：
2014-12-01
影响因子：
2.2
通讯作者：
Xu, Jianzhong
中科院分区：
文献类型：
--
作者：
Xing, Junchao;Jin, Huiyong;Xu, Jianzhong
背景：为了在组织工程学的背景下了解骨缺损愈合的细胞机制，一个可靠、可重复性和标准化的小动物大段骨缺损模型是必不可少的。本研究的目的是建立和评价小鼠双侧股骨缺损模型。材料和方法：取6只小鼠(FVB/N)，取供体小鼠骨髓间充质干细胞(MBMSCs)，植入部分脱钙骨基质支架构建组织工程化骨。共36只GFP(+)小鼠用于造模。将带锁钢丝的钛固定板固定于股骨，在双侧股骨中段造成2 mm长的节段性骨缺损。左侧和右侧股骨缺损处分别植入组织工程化骨和对照支架。愈合过程通过X射线摄影、微型计算机断层摄影和组织学进行监测。用免疫荧光和实时聚合酶链式反应检测移植的mBMSCs对宿主CD31(+)细胞的募集能力。结果：术后除2只小鼠不明原因死亡外，无其他并发症发生。所有动物均实现了股骨和种植体的全负荷稳定固定。由于mBMSCs的引入，骨缺损的修复过程明显加快。结论：本研究建立了一种可行、重复性好、具有临床实用价值的双侧股骨大段骨缺损小鼠模型。该模型可用于骨组织工程领域的基础研究。(C)2014 Elsevier Inc.保留所有权利。
Background: To understand the cellular mechanism underlying bone defect healing in the context of tissue engineering, a reliable, reproducible, and standardized load-bearing large segmental bone defect model in small animals is indispensable. The aim of this study was to establish and evaluate a bilateral femoral defect model in mice.Materials and methods: Donor mouse bone marrow mesenchymal stem cells (mBMSCs) were obtained from six mice (FVB/ N) and incorporated into partially demineralized bone matrix scaffolds to construct tissue-engineered bones. In total, 36 GFP(+) mice were used for modeling. Titanium fixation plates with locking steel wires were attached to the femurs for stabilization, and 2- mm-long segmental bone defects were created in the bilateral femoral midshafts. The defects in the left and right femurs were transplanted with tissue-engineered bones and control scaffolds, respectively. The healing process was monitored by x-ray radiography, microcomputed tomography, and histology. The capacity of the transplanted mBMSCs to recruit host CD31(+) cells was investigated by immunofluorescence and real-time polymerase chain reaction.Results: Postoperatively, no complication was observed, except that two mice died of un-known causes. Stable fixation of femurs and implants with full load bearing was achieved in all animals. The process of bone defect repair was significantly accelerated due to the introduction of mBMSCs. Moreover, the transplanted mBMSCs attracted more host CD31(+) endothelial progenitors into the grafts.Conclusions: The present study established a feasible, reproducible, and clinically relevant bilateral femoral large segmental bone defect mouse model. This model is potentially suitable for basic research in the field of bone tissue engineering. (C) 2014 Elsevier Inc. All rights reserved.