Micronized acellular dermal matrix as an efficient expansion substrate and delivery vehicle of adipose-derived stem cells for vocal fold regeneration
Micronized acellular dermal matrix as an efficient expansion substrate and delivery vehicle of adipose-derived stem cells for vocal fold regeneration
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微粉化脱细胞真皮基质作为脂肪干细胞用于声带再生的有效扩增基质和递送载体
DOI：
10.1002/lary.23330
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发表时间：
2012-08-01
期刊：
影响因子：
2.6
通讯作者：
Jin, Yan
中科院分区：
文献类型：
--
作者：
Liang, Qiang;Liu, Shiyu;Jin, Yan
目的/假设：细胞疗法已被证明可以防止声带瘢痕形成和萎缩。然而，包括大量细胞体外扩增和体内移植细胞存活率较低的问题必须得到解决。本研究的目的是将微粉化的脱细胞真皮基质(MADM)作为兔同种异体脂肪干细胞(ADSCs)的扩增基质，并将其与细胞结合应用于声带再生。研究设计：动物实验。方法：采用3-(4，5-dimethylthizazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfonyl)-2H-tetrazolium，法检测ADSCs在MADM上的增殖情况，并建立兔急性声带激光损伤模型。18只新西兰大白兔随机分为3组，分别注射ADSC-MADM、ADSCs和MADM。喉镜下进行形态分析，苏木精-伊红染色、van Gieson染色和免疫荧光染色显示组织学改变。此外，通过CM-DIL细胞标记检测ADSCs在体内的存活情况。结果：与二维培养相比，MADM能显著促进ADSCs的增殖。形态和组织学分析表明，与仅使用MAMD或未治疗样本相比，使用ADSC-MADM或仅使用ADSCs可成功防止瘢痕形成和萎缩。此外，ADSC-MADM的治疗效果优于单纯使用ADSCs，这可能是由于MADM显著提高了移植的ADSCs的存活率。结论：MADM可作为ADSCs在声带再生中的一种有效的扩增基质和载体。喉镜检查，2012年
Objectives/Hypothesis: Cell therapy has been shown to prevent vocal fold scarring and atrophy. However, problems that include the expansion of large numbers of cells in vitro and the poor survival of transplanted cells in vivo must be solved. The aim of this study was to use micronized acellular dermal matrix (MADM) as an expansion substrate of rabbit allogeneic adipose-derived stem cells (ADSCs) and to apply the combination of the matrix and cells, ADSC-MADM, to vocal fold regeneration. Study Design: Animal experiment. Methods: The proliferation of ADSCs that were cultured on the MADM was evaluated using 3-(4,5-dimethylthizazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfonyl)-2H-tetrazolium, and a rabbit acute vocal fold injury model was established by laser injury. Eighteen New Zealand white rabbits were randomly divided into three groups, which were injected with ADSC-MADM, ADSCs, and MADM, respectively. Morphological analysis was performed by laryngoscope, and histological analyses were indicated by hematoxylin and eosin staining, van Gieson staining, and immunofluorescence. Additionally, the in vivo survival of the ADSCs was determined by CM-Dil cell labeling. Results: When compared with a two-dimensional culture, the MADM significantly promoted proliferation of ADSCs. Morphological and histological analyses indicated that, when compared to only using of MAMD or the nontreatment sample, the use of ADSC-MADM or only using ADSCs successfully prevent scarring and atrophy. Moreover, ADSC-MADM exhibited a better therapeutic effect than when only using ADSCs, which was probably due to the MADM significantly enhancing the survival of transplanted ADSCs. Conclusions: MADM could be used as an efficient expansion substrate and delivery vehicle for ADSCs in vocal fold regeneration. Laryngoscope, 2012