Membrane protein crystallization using laser irradiation
Membrane protein crystallization using laser irradiation
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DOI：
10.1143/jjap.43.l1376
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发表时间：
2004-10-15
期刊：
影响因子：
--
通讯作者：
Sasaki, T
中科院分区：
文献类型：
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作者：
Adachi, H;Murakami, S;Sasaki, T
我们利用飞秒激光照射演示了膜蛋白的结晶。这种方法，我们称之为激光辐照生长技术 (LIGHT)，可用于在低过饱和范围的溶液中生产 AcrB 晶体。 LIGHT 的特点是成核时间减少。这一特征对于结晶膜蛋白很重要，因为它们在溶解为蛋白质洗涤剂胶束时具有不稳定的特性。利用光，获得了膜转运蛋白 AcrB 的高质量晶体。发现所得晶体对于 X 射线衍射具有足够高的分辨率。这里报告的结果表明，光是膜蛋白结晶以及可溶性蛋白生长的强大工具。
We demonstrate the crystallization of a membrane protein using femtosecond laser irradiation. This method, which we call the laser irradiated growth technique (LIGHT), is useful for producing AcrB crystals in a solution of low supersaturation range. LIGHT is characterized by reduced nucleation times. This feature is important for crystallizing membrane proteins because of their labile properties when solubilized as protein-detergent micelles. Using LIGHT, high-quality crystals of a membrane transporter protein, AcrB, were obtained. The resulting crystals were found to be of sufficiently high resolution for X-ray diffraction. The results reported here indicate that LIGHT is a powerful tool for membrane protein crystallization, as well as for the growth of soluble proteins.