4 SECRETORY PROTEINS SYNTHESIZED BY HEPATOCYTES ARE TRANSPORTED FROM ENDOPLASMIC-RETICULUM TO GOLGI-COMPLEX AT DIFFERENT RATES
4 SECRETORY PROTEINS SYNTHESIZED BY HEPATOCYTES ARE TRANSPORTED FROM ENDOPLASMIC-RETICULUM TO GOLGI-COMPLEX AT DIFFERENT RATES
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DOI：
10.1002/j.1460-2075.1984.tb01775.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
PETERSON, PA
中科院分区：
文献类型：
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作者：
FRIES, E;GUSTAFSSON, L;PETERSON, PA
采用脉冲追踪法结合亚细胞分级分离和定量免疫沉淀技术研究了大鼠肝细胞分泌蛋白白蛋白、转铁蛋白、前白蛋白和视黄醇结合蛋白的胞内转运。蛋白质从内质网（ER）运输到高尔基复合体（GC）在很大程度上不同的速率（t1/2 [半衰期] = 14-137分钟），表明这些细胞器之间的分泌蛋白的运输是受选择性的，可能是受体介导的过程，而不是通过大量的相转移。所有蛋白质从高尔基复合体到培养基的转运都是快速的（t1/2 apprx. 15分钟），并可能以相同的速率发生。与这些动力学数据一致，GC组分中快速转运蛋白（白蛋白）的量高（相对于ER组分中的量），而GC组分中缓慢转运蛋白（转铁蛋白）的量低，如放射免疫测定法所测定。
Pulse-chase experiments in conjunction with subcellular fractionation and quantitative immunoprecipitation were used to study the intracellular transport of 4 secretory proteins, albumin, transferrin, prealbumin and retinol-binding protein, in isolated rat hepatocytes. The proteins were transported from the endoplasmic reticulum (ER) to the Golgi complex (GC) at greatly different rates (t1/2 [half-time] = 14-137 min), indicating that transport of secretory proteins between these organelles is effected by a selective, possibly receptor-mediated process and not through bulk phase transfers. The transport from the Golgi complex to the medium was rapid for all proteins (t1/2 .apprx. 15 min) and possibly occurred at the same rate. Consistent with these kinetic data, the amount of a rapidly transported protein (albumin) in the GC fraction was high (relative to its amount in the ER fraction) whereas the amount of a slowly transported protein (transferrin) in the GC fraction was low, as determined by radioimmunoassays.