Proliferation and triglyceride synthesizing activities of fibroblast-like cells derived from epididymal and subcutaneous adipose tissues of rats.
Proliferation and triglyceride synthesizing activities of fibroblast-like cells derived from epididymal and subcutaneous adipose tissues of rats.
复制标题
大鼠附睾和皮下脂肪组织来源的成纤维细胞样细胞的增殖和甘油三酯合成活性。
DOI：
10.1080/00365519309088413
复制
发表时间：
1993
影响因子：
2.1
通讯作者：
S. Yoshida
中科院分区：
文献类型：
--
作者：
H. Inadera;Y. Ishikawa;K. Shirai;Y. Saito;S. Yoshida
从大鼠皮下脂肪组织和附睾脂肪组织中制备成纤维细胞样细胞，并研究其增殖、[14C]脱氧葡萄糖摄取和[14C]棕榈酸酯的甘油三酯合成。来自皮下脂肪组织的成纤维细胞样细胞的增殖大于来自附睾脂肪组织的成纤维细胞样细胞的增殖。胰岛素增强来自皮下脂肪组织的成纤维细胞样细胞的增殖，但不增强来自附睾脂肪组织的成纤维细胞样细胞的增殖。两种来源的成纤维细胞样细胞对[14C]脱氧葡萄糖的摄取相似。附睾成纤维细胞样细胞从[14C]棕榈酸酯合成甘油三酯高于皮下成纤维细胞样细胞。两种来源的成纤维细胞样细胞对[14C]脱氧葡萄糖的摄取和从[14C]棕榈酸酯的甘油三酯的合成并未因添加胰岛素而增强。这些发现表明皮下和附睾脂肪组织中存在不同类型的脂肪细胞增殖，并表明皮下脂肪组织中成纤维细胞样细胞的增殖受到胰岛素的调节。来自两种脂肪组织的未成熟脂肪细胞的甘油三酯合成不受胰岛素影响。
Fibroblast-like cells from subcutaneous adipose tissue and epididymal adipose tissue were prepared from rats and their proliferation and [14C]deoxy-glucose uptake and triglyceride synthesis from [14C]palmitate were investigated. The proliferation of fibroblast-like cells from subcutaneous adipose tissue was greater than that from epididymal adipose tissue. Insulin enhanced the proliferation of fibroblast-like cells derived from subcutaneous adipose tissue, but not from epididymal adipose tissue. The uptake of [14C]deoxy-glucose by fibroblast-like cells from the two sources was similar. Triglyceride synthesis from [14C]palmitate by epididymal fibroblast-like cells was higher than that by subcutaneous fibroblast-like cells. The [14C]deoxy-glucose uptake and triglyceride synthesis from [14C]palmitate by fibroblast-like cells from the two sources were not enhanced by the addition of insulin. These findings suggest the existence of different types of adipocytes in subcutaneous and epididymal adipose tissues in terms of proliferation, and show that the proliferation of fibroblast-like cells from subcutaneous adipose tissue is regulated by insulin. The triglyceride synthesis by immature adipocytes from both adipose tissues was not affected by insulin.