INCREASE IN TYPE-1 AND TYPE-3 COLLAGENS IN HUMAN ALCOHOLIC LIVER CIRRHOSIS
INCREASE IN TYPE-1 AND TYPE-3 COLLAGENS IN HUMAN ALCOHOLIC LIVER CIRRHOSIS
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DOI：
10.1073/pnas.73.2.539
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
MARTINEZPALOMO, A
中科院分区：
文献类型：
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作者：
ROJKIND, M;MARTINEZPALOMO, A
从正常人的肝脏和酒精性肝硬化患者的肝脏中以约30%的产率分离出大量胶原。经化学分析和电子显微镜检查，每种情况下的胶原蛋白由2种类型组成，与皮肤的I型和III型胶原蛋白相同或相似。正常肝脏胶原主要为I型，而炎性肝胶原则由两种类型的胶原组成。使用羧甲基纤维素色谱法，来自异位肝脏的I型胶原蛋白显示出一条α 2链和两条α 1链。店α 1链彼此可分离，但用CNBr处理后从它们获得的肽的凝胶电泳图谱几乎相同，并且类似于用大鼠皮肤I型胶原α 1链的CNBr肽获得的图谱。这两种类型的胶原蛋白的增加是部分原因，观察到的变形的结构的肝硬化与增加刚度的基质。III型胶原蛋白的优势，在该地区的崩溃的架构，如其他人所示，很少有成纤维细胞存在，表明肝细胞可能有一个重要的功能，在肝硬化过程中的纤维化。
Collagen in bulk was isolated in about 30% yield from the livers of normal human beings and from livers of persons with alcoholic cirrhosis. Analyzed chemically and examined by electron microscopy, the collagen in each case consisted of 2 types identical with, or resembling closely, type I and type III collagens of skin. The collagen from normal liver was predominantly type I, whereas that from cirrhotic livers consisted of approximately equal amounts of the 2 types. Using carboxymethyl-cellulose chromatography, the type I collagen from the cirrhotic livers showed one .alpha.2 chain and two .alpha.1 chains. chains. The .alpha.1 chains were separable from one another, but gel electrophoretic patterns of peptides obtained from them after treatment with CNBr were almost identical, and resembled the pattern obtained with CNBr peptides of the .alpha.1 chain of rat skin type I collagen. The increased collagen of both types was responsible in part for the observed distortion of the architecture of the cirrhotic livers associated with increased rigidity of the stroma. The predominance of type III collagen in the areas of collapse of architecture where, as shown by others, few fibroblasts are present, suggests that hepatocytes might have an important function in fibrogenesis during the course of liver cirrhosis.