New insights in flat oyster Ostrea edulis resistance against the parasite Bonamia ostreae
New insights in flat oyster Ostrea edulis resistance against the parasite Bonamia ostreae
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DOI：
10.1016/j.fsi.2012.01.026
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发表时间：
2012-06-01
影响因子：
4.7
通讯作者：
Arzul, I.
中科院分区：
文献类型：
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作者：
Morga, B.;Renault, T.;Arzul, I.
在欧洲，由寄生虫Bonamia ostrae引起的Bonamiosis与扁平牡蛎种群的大量死亡有关。目前，控制这种疾病依赖于疾病管理做法和限制转移。以前，大规模的选择是基于对B感染的存活率。牡蛎已被用于生产具有抗性后代的扁平牡蛎家族。在试图了解参与抗病性的分子机制，差异表达的序列标签之间的抗性和野生牡蛎血细胞，都感染了寄生虫，使用抑制消减杂交。使用定量逆转录酶PCR研究了7个EST的表达。细胞外超氧化物歧化酶、细胞凋亡抑制剂（OeIAP）、Fas配体（OeFas-配体）和组织蛋白酶B的基线表达显著增加，而亲环素B在抗性牡蛎中出现显著降低。考虑到OeFas配体和OeIAP的开放阅读框架对进一步研究的巨大兴趣，我们对OeFas配体和OeIAP的开放阅读框架进行了完全测序。(C)2012爱思唯尔有限公司保留所有权利。
Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality in flat oyster stocks in Europe. Control of the disease currently relies on disease management practices and transfer restriction. Previously, massal selections based on survival to challenge to infection with B. ostreae have been applied to produce flat oyster families with resistant progeny. In an attempt to understand the molecular mechanisms involved in disease resistance, differentially expressed sequence tags between resistant and wild Ostrea edulis haemocytes, both infected with the parasite, were identified using suppression subtractive hybridisation. Expression of seven ESTs has been studied using quantitative reverse-transcriptase PCR. The base-line expression of an extracellular superoxide dismutase, inhibitor of apoptosis (OeIAP), Fas ligand (OeFas-ligand) and Cathepsin B was significantly increased, whilst cyclophilin B appeared significantly decreased in resistant oysters. Considering their great interest for further studies, the open reading frames of the OeFas-ligand and OeIAP were completely sequenced. (C) 2012 Elsevier Ltd. All rights reserved.