Anti-inflammatory and antioxidant activities of the nonlipid (aqueous) components of sesame oil: potential use in atherosclerosis.
Anti-inflammatory and antioxidant activities of the nonlipid (aqueous) components of sesame oil: potential use in atherosclerosis.
复制标题
芝麻油非脂质（水性）成分的抗炎和抗氧化活性：在动脉粥样硬化中的潜在用途。
DOI：
10.1089/jmf.2014.0139
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发表时间：
2015
影响因子：
2.4
通讯作者：
Parthasarathy,Sampath
中科院分区：
文献类型：
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作者：
Selvarajan,Krithika;Narasimhulu,ChandrakalaAluganti;Bapputty,Reena;Parthasarathy,Sampath
近年来，饮食干预以预防炎症和动脉粥样硬化一直是一个主要焦点。我们之前报道过芝麻油（SO）可有效抑制低密度脂蛋白受体阴性小鼠的动脉粥样硬化。我们还注意到，许多促炎标志物的水平在SO治疗的动物中较低。在这项研究中，我们测试了与SO相关的非脂质水性成分是否具有抗炎和抗氧化作用。聚合酶链反应阵列数据表明，芝麻油水提取物（SOAE）是有效地减少脂多糖（LPS）诱导的RAW 264.7巨噬细胞的炎症。在用SOAE预处理的细胞中，还独立分析了炎性细胞因子如白细胞介素（IL）-1α、IL-6和肿瘤坏死因子α（TNF-α）的表达，随后进行炎性攻击。在人脐静脉内皮细胞中检测SOAE对TNF-α诱导的MCP-1和VCAM 1表达的影响。我们观察到SOAE以浓度依赖性方式显著降低巨噬细胞和内皮细胞中的炎症标志物。不同浓度的SOAE对LPS诱导的TNF-α和IL-6水平也有明显的抑制作用。我们还注意到，在SOAE的存在下，NF-κ B的转录和易位受到抑制。SOAE在体外对脂蛋白的氧化也有抑制作用，提示SOAE中含有有效的抗炎和抗氧化成分。此外，SOAE差异调节清道夫受体的表达，并通过激活肝脏X受体（LXRs）增加ATP结合盒A1（ABCA 1）mRNA的表达，这表明对脂质代谢的额外影响。因此，SOAE似乎是多能的，并可能作为一个有价值的非药物剂在动脉粥样硬化和其他炎症性疾病。
Dietary intervention to prevent inflammation and atherosclerosis has been a major focus in recent years. We previously reported that sesame oil (SO) was effective in inhibiting atherosclerosis in low-density lipoprotein-receptor negative mice. We also noted that the levels of many proinflammatory markers were lower in the SO-treated animals. In this study we tested whether the non-lipid, aqueous components associated with SO would have anti-inflammatory and antioxidant effects. Polymerase chain reaction array data indicated that sesame oil aqueous extract (SOAE) was effective in reducing lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophage cells. Expression of inflammatory cytokines such as interleukin (IL)-1α, IL-6, and tumor necrosis factorα(TNF-α) was also analyzed independently in cells pretreated with SOAE followed by inflammatory assault. Effect of SOAE on TNF-α-induced MCP-1 and VCAM1 expression was also tested in human umbilical vein endothelial cells. We observed that SOAE significantly reduced inflammatory markers in both macrophages and endothelial cells in a concentration-dependent manner. SOAE was also effective in inhibiting LPS-induced TNF-αand IL-6 levelsin vivoat different concentrations. We also noted that in the presence of SOAE, transcription and translocation of NF-kappaB was suppressed. SOAE was also effective in inhibiting oxidation of lipoproteinsin vitro.These results suggest the presence of potent anti-inflammatory and antioxidant compounds in SOAE. Furthermore, SOAE differentially regulated expression of scavenger receptors and increased ATP-binding cassette A1 (ABCA1) mRNA expression by activating liver X receptors (LXRs), suggesting additional effects on lipid metabolism. Thus, SOAE appears multipotent and may serve as a valuable nonpharmacological agent in atherosclerosis and other inflammatory diseases.