IMMUNOCYTOCHEMICAL STUDY OF ANTI-MULLERIAN HORMONE IN SHEEP OVARIAN FOLLICLES DURING FETAL AND POSTNATAL-DEVELOPMENT
IMMUNOCYTOCHEMICAL STUDY OF ANTI-MULLERIAN HORMONE IN SHEEP OVARIAN FOLLICLES DURING FETAL AND POSTNATAL-DEVELOPMENT
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DOI：
10.1530/jrf.0.0800509
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发表时间：
1987-07-01
期刊：
影响因子：
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通讯作者：
JOSSO, N
中科院分区：
文献类型：
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作者：
BEZARD, J;VIGIER, B;JOSSO, N
用抗反刍动物抗苗勒氏激素(AMH)的单抗，用亲和素-生物素免疫组织化学方法检测了围产期和出生后绵羊卵巢中的AMH。免疫反应仅限于颗粒细胞，并受卵泡发育程度和动物年龄的影响。在胎儿中，只有最高级的卵泡在怀孕120天时显示出微弱的免疫反应，在年轻的动物中没有观察到反应。出生前和出生后即刻，原始卵泡仍为阴性，但在年轻的生长卵泡中诱导出微弱的反应，并随着卵泡大小的增加而增加。在有腔卵泡，尤其是最内侧的颗粒细胞层，靠近卵母细胞并排列在窦腔内的卵泡内可见强免疫反应。
Anti-Mullerian hormone (AMH) was detected in perinatal and postnatal sheep ovaries, using avidin-biotin immunohistochemistry with a monoclonal antibody specific for ruminant AMH. Immunoreactivity was limited to granulosa cells, and was influenced both by the degree of follicular development, and by the age of the animal. In the fetus, only the most advanced follicles exhibited a faint immunoreactivity at 120 days gestation, and no reaction was observed in younger animals. Immediately before and after birth, primordial follicles were still negative, but a faint reaction was elicited in young growing follicles, increasing with follicle size. Strong immunoreactivity was visible in antral follicles, especially in the innermost granulosa cell layers, close to the oocyte and lining the antral cavity.