Human striatal dopamine receptors are organized in compartments.
Human striatal dopamine receptors are organized in compartments.
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人类纹状体多巴胺受体被组织成隔室。
DOI：
10.1073/pnas.83.20.8002
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发表时间：
1986
影响因子：
11.1
通讯作者：
Marshall,JF
中科院分区：
文献类型：
--
作者：
Joyce,JN;Sapp,DW;Marshall,JF
通过[3H]螺哌啶醇结合的定量放射自显影在死后人类纹状体中可视化的多巴胺（D2）受体被组织成低受体密度的限制区域，通过较高D2密度的区域与其他此类区域分开。尾状核和壳核富含 D2 的区域的结合量是缺乏 D2 区域的两倍。希尔系数是从[3H]螺哌啶醇与人类纹状体薄切片的结合饱和度分析中获得的，其值接近一致，表明结合发生在单一类型的位点上。 [3H]螺哌啶醇结合的斑块状未因温育后从组织切片中去除脂质而改变，这表明白质和灰质中氚的吸收差异并不能解释不均匀分布。富含 D2 和缺乏 D2 的区域似乎形成了沿前后轴定向的迷宫，并且通常分别与富含乙酰胆碱酯酶和缺乏乙酰胆碱酯酶的区室对齐，如在染色的相邻切片上可见的。因此，多巴胺 D2 受体的分布符合人类尾壳核的“纹状体”组织，这一发现表明这种受体亚型可能介导多巴胺对该结构内不同神经化学区室的影响。
Dopamine (D2) receptors visualized in postmortem human striatum by quantitative autoradiography of [3H]spiroperidol binding are organized into circumscribed zones of low receptor density separated from other such zones by regions of higher D2 density. The D2-rich zones of the caudate nucleus and putamen contain twice the binding of D2-poor zones. The Hill coefficient, obtained from saturation analysis of [3H]spiroperidol binding to thin sections of human striatum, gave a value near unity, indicating the binding was occurring to a single type of site. The patchiness of [3H]spiroperidol binding was unaltered by postincubation removal of lipid from the tissue sections, indicating that a differential absorption of tritium in white and grey matter does not account for the heterogeneous distribution. The D2-rich and D2-poor regions appear to form labyrinths oriented in the anterior-posterior axis and are typically aligned with, respectively, acetylcholinesterase-rich and -poor compartments as visualized on stained adjacent sections. Thus, the distribution of dopamine D2 receptors conforms to the "striosomal" organization of the human caudate-putamen, a finding that suggests that this receptor subtype may mediate the influence of dopamine on distinct neurochemical compartments within the structure.