A web-based interactive developmental table for the ascidian Ciona intestinalis, including 3D real-image embryo reconstructions:: I.: From fertilized egg to hatching larva
A web-based interactive developmental table for the ascidian Ciona intestinalis, including 3D real-image embryo reconstructions:: I.: From fertilized egg to hatching larva
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DOI：
10.1002/dvdy.21188
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发表时间：
2007-07-01
影响因子：
2.5
通讯作者：
Ikeo, Kazuho
中科院分区：
文献类型：
--
作者：
Hotta, Kohji;Mitsuhara, Kenta;Ikeo, Kazuho
海鞘脊索动物玻璃海鞘是一种已建立的模式生物，经常被用来研究细胞发育，也是一种迅速崛起的模式生物，具有很强的发育系统生物学研究潜力。然而，对于这种生物体没有标准化的发育表。在这项研究中，我们提出了标准的基于网络的图像资源称为FABA：四维海鞘体图集，包括海鞘的三维（3D）和横截面图像，通过发展的时间过程。这些图像是从共聚焦激光扫描显微镜（CLSM）收集的3,000多张高分辨率真实的图像重建的，这些图像是在新定义的26个不同的发育阶段（阶段1-26）从受精卵到孵化幼虫，分为6个时期，即受精卵，卵裂，原肠胚，神经胚，尾芽和幼虫时期。我们的数据集将有助于标准化的发展阶段的形态比较，以及为几个功能研究的身体计划在脊索动物提供指导方针。发展动力学236：1790-1805，2007年。(c)2007 Wiley-Liss，Inc.
The ascidian chordate Ciona intestinalis is an established model organism frequently exploited to examine cellular development and a rapidly emerging model organism with a strong potential for developmental systems biology studies. However, there is no standardized developmental table for this organism. In this study, we made the standard web-based image resource called FABA: Four-dimensional Ascidian Body Atlas including ascidian's three-dimensional (3D) and cross-sectional images through the developmental time course. These images were reconstructed from more than 3,000 high-resolution real images collected by confocal laser scanning microscopy (CLSM) at newly defined 26 distinct developmental stages (stages 1-26) from fertilized egg to hatching larva, which were grouped into six periods named the zygote, cleavage, gastrula, neurula, tailbud, and larva periods. Our data set will be helpful in standardizing developmental stages for morphology comparison as well as for providing the guideline for several functional studies of a body plan in chordate. Developmental Dynamics 236:1790-1805, 2007. (c) 2007 Wiley-Liss, Inc.