High-efficiency translational bypassing of non-coding nucleotides specified by mRNA structure and nascent peptide
High-efficiency translational bypassing of non-coding nucleotides specified by mRNA structure and nascent peptide
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DOI：
10.1038/ncomms5459
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发表时间：
2014-07-01
影响因子：
16.6
通讯作者：
Rodnina, Marina V.
中科院分区：
文献类型：
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作者：
Samatova, Ekaterina;Konevega, Andrey L.;Rodnina, Marina V.
噬菌体T4的基因产物60（gp60）作为单链从不连续的阅读框合成，这是核糖体绕过50个核苷酸的非编码mRNA区的结果。为了确定旁路所需的最小信号集，我们概括了有效的翻译旁路在体外重组的翻译系统从大肠杆菌。我们发现，信号，促进有效和准确的旁路，指定的基因60 mRNA序列。mRNA的系统分析表明，非编码区的上游和下游序列以及新生肽的意外贡献。在旁路过程中，核糖体以进行性方式在mRNA轨道上向前滑动。滑动可能不仅对gp60合成有作用，而且在起始期间的阅读框选择或延伸期间的tRNA易位的常规mRNA翻译期间也有作用。
The gene product 60 (gp60) of bacteriophage T4 is synthesized as a single polypeptide chain from a discontinuous reading frame as a result of bypassing of a non-coding mRNA region of 50 nucleotides by the ribosome. To identify the minimum set of signals required for bypassing, we recapitulated efficient translational bypassing in an in vitro reconstituted translation system from Escherichia coli. We find that the signals, which promote efficient and accurate bypassing, are specified by the gene 60 mRNA sequence. Systematic analysis of the mRNA suggests unexpected contributions of sequences upstream and downstream of the non-coding gap region as well as of the nascent peptide. During bypassing, ribosomes glide forward on the mRNA track in a processive way. Gliding may have a role not only for gp60 synthesis, but also during regular mRNA translation for reading frame selection during initiation or tRNA translocation during elongation.