Loss of circadian clock function decreases reproductive fitness in males of Drosophila melanogaster
Loss of circadian clock function decreases reproductive fitness in males of Drosophila melanogaster
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DOI：
10.1073/pnas.032426699
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发表时间：
2002-02-19
影响因子：
11.1
通讯作者：
Giebultowicz, JM
中科院分区：
文献类型：
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作者：
Beaver, LM;Gvakharia, BO;Giebultowicz, JM
生命功能的昼夜节律协调被认为有助于有机体的健康；然而，这种贡献尚未在任何动物身上得到令人信服的证明。适应度最重要的衡量标准是个体和物种的繁殖产出。在这里，我们研究了时钟功能丧失对具有突变的 period (per(0)、timeless (tim(0))、cycle (cyc(0)) 和 Clock (Clk(Irk)) 基因的果蝇生殖适应性的影响。具有时钟缺陷表型的夫妇之间的单交导致与野生型果蝇相比，后代减少了大约 40%，因为产卵数量减少，未受精率更高。与野生型雌性交配的 per(0) 和 tim(0) 雄性果蝇的生殖能力下降证明了雄性对这种表型的贡献。通过逆转具有功能性时钟的果蝇的低生育力表型，证实了时钟基因从睾丸释放到精囊的精子数量显着下降。通过结合分子和生理学方法，我们确定了生殖系统中的生物钟，并确定了其在促进黑腹果蝇生殖健康的精子释放中的作用。
Circadian coordination of life functions is believed to contribute to an organism's fitness; however, such contributions have not been convincingly demonstrated in any animal. The most significant measure of fitness is the reproductive output of the individual and species. Here we examined the consequences of loss of clock function on reproductive fitness in Drosophila melanogaster with mutated period (per(0), timeless (tim(0)), cycle (cyc(0)), and Clock (Clk(Irk)) genes. Single mating among couples with clock-deficient phenotypes resulted in approximate to40% fewer progeny compared with wild-type flies, because of a decreased number of eggs laid and a greater rate of unfertilized eggs. Male contribution to this phenotype was demonstrated by a decrease in reproductive capacity among per(0) and tim(0) males mated with wild-type females. The important role of clock genes for reproductive fitness was confirmed by reversal of the low-fertility phenotype in flies with rescued per or tim function. Males lacking a functional clock showed a significant decline in the quantity of sperm released from the testes to seminal vesicles, and these tissues displayed rhythmic and autonomous expression of clock genes. By combining molecular and physiological approachesz, we identified a circadian clock in the reproductive system and defined its role in the sperm release that promotes reproductive fitness in D. melanogaster.