Soil nematode abundance and diversity from four vegetation types in Central Mexico
Soil nematode abundance and diversity from four vegetation types in Central Mexico
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DOI：
10.1163/15685411-00003119
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发表时间：
2018-01-01
期刊：
影响因子：
1.2
通讯作者：
Mejia-Madrid, Hugo H.
中科院分区：
文献类型：
--
作者：
Mejia-Madrid, Hugo H.
土壤线虫丰度和MOTU多样性估计从墨西哥的地理广阔的地区，其中包括四个公认的七种植被类型。植被类型的线虫群落的丰度和多样性进行了评估，并推断它们之间的生态关系。2013年、2014年和2015年，对热带雨林、热带干燥落叶林、温带针叶林和旱生灌木进行了土壤采样。14个采样点，约10-20个样本，每个网站从11个地点分布在墨西哥中部进行了评估。取样的海拔高度从海拔113米到113米不等。（热带沿海平原）到海拔2400米（Trans-Mexican Volcanic Belt）从每个采样点的保护区和耕地中抽取样本，采样点的面积大于等于100平方米（2）。共收集了来自25个已鉴定的线虫科的13263个个体。科多度指数和样地间的互补性指数在一定程度上反映了植被类型间的亲缘关系。然而，统计分析显示，线虫家族丰度之间没有差异的网站，只有家庭之间在所有网站。分子操作分类单位（MOTU）方法被用作生物多样性评价的框架。从这些，77个高质量的序列分类条码回收，后来确定与形态特征。只有6个序列与GenBank中报道的序列在98-99%的水平上匹配。结果表明，在41个MOTU序列中，热带雨林、热带干落叶林和旱生灌木的MOTU序列的截止遗传一致性均大于3%，温带针叶林和受干扰温带针叶林的MOTU序列的截止遗传一致性分别为73%和70%。此外，12.2%的MOTU在地方之间共享，87.8%表现出明显的地方性分布。从一个小样本的MOTU多样性，揭示了相当多的线虫多样性的潜力，进行了讨论。
Soil nematode abundance and MOTU diversity were estimated from a geographically broad area of Mexico that included four out of seven recognised vegetation types. Vegetation types were assessed for abundance and diversity of nematode communities and inferred ecological relationships between them. Soils were sampled from tropical rainforest, tropical dry deciduous forest, temperate coniferous forest and xerophytic shrub during 2013, 2014 and 2015. Fourteen sampling sites with ca 10-20 samples per site from 11 localities spread across Central Mexico were assessed. Altitudes sampled ranged from 113 m a.s.l. (tropical coastal plain) to 2400 m a.s.l. (Trans-Mexican Volcanic Belt). Samples were drawn from conserved and cultivated plots from each sampling site covering an area of >= 100 m(2). A total of 13 263 individuals from 25 identified families of nematodes were collected. Family abundance and complementarity indices between sites revealed to some extent the affinities between vegetation types. Nevertheless, statistical analyses revealed no differences between nematode family abundances between sites, only between families across all sites. Molecular operational taxonomic units (MOTU) methods were employed as a framework to assess biodiversity. From these, 77 high-quality sequences for taxonomic barcoding were recovered and later identified with morphological traits. Only six sequences matched at a 98-99% level with those reported in GenBank. Sequences amounted to a total of 41 MOTU, where 100% of the MOTU from both conserved and disturbed tropical rainforest, tropical dry deciduous forest and xerophytic shrub exhibited a >= 3% cut-off genetic identity, whilst temperate coniferous forest and disturbed temperate coniferous forest showed 73% and 70% respectively. In addition, 12.2% MOTU were shared among localities and 87.8% exhibited an apparently locality-limited distribution. The potential for a considerable diversity of nematodes, as revealed from a small sample of MOTU diversity, is discussed.