Antigenic stimulation regulates the level of expression of interleukin 2 receptor on human T cells.
Antigenic stimulation regulates the level of expression of interleukin 2 receptor on human T cells.
复制标题
抗原刺激调节人 T 细胞上白细胞介素 2 受体的表达水平。
DOI：
10.1073/pnas.81.7.2172
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
Strominger,JL
中科院分区：
文献类型：
--
作者：
Hemler,ME;Brenner,MB;McLean,JM;Strominger,JL
利用抗原特异性、白细胞介素 2 (IL-2) 依赖性人类 T 细胞系和克隆来研究 IL-2 受体表达与抗原刺激之间的关系。 2周或更长时间未接触抗原的T细胞表达稳定的低水平IL-2受体。再次暴露于抗原后，IL-2受体的水平迅速增加10至30倍，IL-2受体表达的峰值水平出现在15-30小时。该峰值先于细胞增殖[( 3H]胸苷掺入)的峰值，该峰值位于 48-72 小时。 IL-2 受体表达达到峰值后 2-14 天内，它恢复到较低的基线水平。 IL-2受体水平的短暂升高是抗原特异性的，因为它发生在对特定同种异体刺激细胞的反应中，而不是在暴露于表达不相关HLA同种异型的细胞后。其他细胞表面蛋白的水平，包括与 T 细胞激活相关的蛋白（HLA-DR、T10、4F2、A-1A5）以及 T3（被认为是抗原 T 细胞受体复合物的组成部分）的水平，不会因抗原暴露或剥夺而发生变化。由于 IL-2 在整个实验过程中始终保持在高水平，因此抗原诱导的 IL-2 受体变化似乎与 IL-2 本身诱导的变化无关。克隆的 T 细胞和含有 T4 和 T8 亚群的混合群体都显示出相似的 IL-2 受体反应性，表明这一发现适用于大多数（如果不是全部）抗原反应性 T 细胞。
Antigen-specific, interleukin 2 (IL-2)-dependent human T-cell lines and clones were utilized to study the relationship between IL-2 receptor expression and antigenic stimulation. T cells that had not been exposed to antigen for 2 wk or more expressed a stable low level of the IL-2 receptor. After reexposure to antigen, a 10- to 30-fold increase in the level of the IL-2 receptor was rapidly induced, with the peak level of IL-2 receptor expression occurring at 15-30 hr. This peak preceded the peak in cell proliferation [( 3H]thymidine incorporation), which was at 48-72 hr. Within 2-14 days after peak IL-2 receptor expression, it returned to a low base-line level. The transient elevation in IL-2 receptor level was antigen specific because it occurred in response to specific allogeneic stimulator cells but not after exposure to cells expressing irrelevant HLA allotypes. The levels of other cell-surface proteins, including those related to T-cell activation (HLA-DR, T10, 4F2, A-1A5) as well as T3, which has been proposed to be a component of the T-cell receptor complex for antigen, did not change in response to antigen exposure or deprivation. Because IL-2 was maintained at a consistently high level throughout these experiments, the antigen-induced changes in the IL-2 receptor appear to be independent of changes induced by IL-2 itself. Both cloned T cells and mixed populations containing T4 and T8 subsets showed similar IL-2 receptor responsiveness, indicating that this finding is generalizable to most, if not to all, antigen-responsive T cells.