SYSTEMIC DELIVERY OF HUMAN GROWTH-HORMONE BY INJECTION OF GENETICALLY ENGINEERED MYOBLASTS
SYSTEMIC DELIVERY OF HUMAN GROWTH-HORMONE BY INJECTION OF GENETICALLY ENGINEERED MYOBLASTS
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DOI：
10.1126/science.1962213
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发表时间：
1991-12-06
期刊：
影响因子：
56.9
通讯作者：
BLAU, HM
中科院分区：
文献类型：
--
作者：
DHAWAN, J;PAN, LC;BLAU, HM
用逆转录病毒载体将编码人生长激素（hGH）的重组基因稳定导入培养的成肌细胞。将基因工程成肌细胞注射到小鼠肌肉中后，血清中可检测到hGH，持续3个月。通过用两种逆转录病毒载体（一种编码hGH，另一种编码来自大肠杆菌的β-半乳糖苷酶）共感染细胞来评估注射的成肌细胞的命运。这些结果提供的证据表明，成肌细胞，它可以融合到预先存在的多核肌纤维的血管化和神经支配，可能是有利的重组蛋白的全身递送的车辆。
A recombinant gene encoding human growth hormone (hGH) was stably introduced into cultured myoblasts with a retroviral vector. After injection of genetically engineered myoblasts into mouse muscle, hGH could be detected in serum for 3 months. The fate of injected myoblasts was assessed by coinfecting the cells with two retroviral vectors, one encoding hGH and the other encoding beta-galactosidase from Escherichia coli. These results provide evidence that myoblasts, which can fuse into preexisting multinucleated myofibers that are vascularized and innervated, may be advantageous as vehicles for systemic delivery of recombinant proteins.