Free energy potential for aggregation of erythrocytes and phosphatidylcholine/phosphatidylserine vesicles in Dextran (36,500 MW) solutions and in plasma.
Free energy potential for aggregation of erythrocytes and phosphatidylcholine/phosphatidylserine vesicles in Dextran (36,500 MW) solutions and in plasma.
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右旋糖酐 (36,500 MW) 溶液和血浆中红细胞和磷脂酰胆碱/磷脂酰丝氨酸囊泡聚集的自由能潜力。
DOI：
10.1016/s0006-3495(83)84297-0
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发表时间：
1983
影响因子：
3.4
通讯作者：
Kukan,B
中科院分区：
文献类型：
--
作者：
Evans,E;Kukan,B
通过测量红细胞表面包封囊泡的程度，对葡聚糖溶液和血浆中人红细胞和脂质囊泡聚集的自由能势（亲和力）进行了定量。每单位面积的接触形成的自由能减少（亲和力）是通过观察平衡时的包封程度来计算的，使用的关系是基于红细胞膜变形粘附到囊泡表面时的弹性顺应性。使用显微操作方法选择单个脂质囊泡（直径2-3 × 10（-4）cm），并将其从含有囊泡悬浮液的腔室转移到显微镜载物台上的单独腔室中，该腔室含有含葡聚糖的EDTA缓冲液中的红细胞或全血浆。将囊泡和红细胞紧密接触，并允许在不迫使细胞聚集在一起的情况下发生接触。为了评估表面电荷密度和空间相互作用对聚集的影响，囊泡由卵磷脂（PC）和牛磷脂酰丝氨酸（PS）的摩尔比（PC/PS）从（1：0）到（1：1）的范围内的混合物制成;囊泡通过在缓冲液中再水化形成。葡聚糖溶液用36，500 MW的锐切级分制备，浓度范围为0- 10重量%，以克（wt/wt）计。（250字处删节）
The free energy potential (affinity) for aggregation of human red blood cells and lipid vesicles in Dextran solutions and blood plasma has been quantitated by measuring to what extent a vesicle is encapsulated by the red cell surface. The free energy reduction per unit area of contact formation (affinity) was computed from the observation of the fractional extent of encapsulation at equilibrium with the use of a relation based on the elastic compliance of the red cell membrane as it is deformed to adhere to the vesicle surface. Micromanipulation methods were used to select and transfer single lipid vesicles (2–3 X 10(-4) cm diameter) from a chamber that contained the vesicle suspension to a separate chamber on the microscope stage that contained red cells in an EDTA buffer with Dextran or whole plasma. The vesicle and a red cell were maneuvered into close proximity and contact allowed to take place without forcing the cells together. To evaluate the effects of surface charge density and steric interactions on aggregation, vesicles were made from mixtures of egg phosphatidylcholine (PC) and bovine phosphatidylserine (PS) over a range of mole ratios (PC/PS)from (1:0) to (1:1); the vesicles were formed by rehydration in buffer. The Dextran solutions were made with a sharp-cut fraction of 36,500 MW in a concentration range of 0–10% by weight in grams (wt/wt).(ABSTRACT TRUNCATED AT 250 WORDS)