Thrombin regulates CD40 expression in microglial cells.
Thrombin regulates CD40 expression in microglial cells.
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凝血酶调节小胶质细胞中 CD40 的表达。
DOI：
10.1097/wnr.0b013e3282fdf4e7
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发表时间：
2008
期刊：
影响因子：
1.7
通讯作者：
Möller,Thomas
中科院分区：
文献类型：
--
作者：
Weinstein,JonathanR;Ettinger,RussellE;Zhang,Matthew;Andersen,Henrik;Hanisch,Uwe-Karsten;Möller,Thomas
小胶质细胞是中枢神经系统的先天免疫细胞，通过增殖、释放细胞因子和增加细胞表面抗原的表达来对损伤做出快速反应。凝血酶是一种多功能丝氨酸蛋白酶，具有激活小胶质细胞的功能。在这里，我们报告了药用级凝血酶剂量依赖性地增加N9小胶质细胞CD40的表达。这种作用被凝血酶抑制剂阻断，被凝血酶受体激活肽模拟，并被丝裂原激活的蛋白激酶途径抑制剂修饰。凝血酶诱导的CD40调节可能在多发性硬化症或中风等血脑屏障破裂的疾病中发挥作用。
Microglial cells are the innate immune cells of the central nervous system and quickly respond to injury by proliferation, cytokine release, and increased cell surface antigen expression. Thrombin is a multifunctional serine proteinase, which has the capability to activate microglial cells. Here, we report that pharmaceutical-grade thrombin dose-dependently increases the expression of CD40 in N9 microglial cells. This effect is blocked by a thrombin inhibitor, mimicked by thrombin receptor-activating peptide and modified by mitogen-activated protein kinase pathway inhibitors. Thrombin-induced CD40 regulation might play a role in diseases with breakdown of the blood–brain barrier such as multiple sclerosis or stroke.