COMPARISON OF BIPHASIC AND MONOPHASIC DEFIBRILLATION WAVE-FORMS IN AN ISOLATED RABBIT HEART PREPARATION
COMPARISON OF BIPHASIC AND MONOPHASIC DEFIBRILLATION WAVE-FORMS IN AN ISOLATED RABBIT HEART PREPARATION
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DOI：
10.1093/cvr/25.12.979
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发表时间：
1991-12-01
影响因子：
10.8
通讯作者：
MCCULLOCH, RM
中科院分区：
文献类型：
--
作者：
HOLLEY, LK;MCCULLOCH, RM
研究目的-目的是开发一个Langendorff兔心脏模型，并比较单相和双相除颤pulses.Design -心脏灌注Krebs-Henseleit解决方案和两个1.4平方厘米的铂铱网状贴片电极缝合到心室。5 ms单相或10 ms双相脉冲，随机选择30、50、70、90、110或130 V的电压，在10 s的纤颤后对心脏进行除颤。测量和主要结果-在每种制备中进行总共72次纤颤和除颤序列。通过逻辑回归分析将结果拟合为S形剂量-反应曲线。50%和80%成功率下拟合数据的电压和能量值（V50，V80，E50，E80）表明，（p < 0.05）双相波形的除颤阈值电压和能量[V50 = 48（SD 19）V，V80 = 87（27）V，E50 = 0.15（0.12）J，E80 = 0.48（0.29）J]与单相波形[V50 = 79（20）V，V80 = 110（20）V，E50 = 0.27（0.12）J，E80 = 0.5（0.12）J]相比。有没有观察到任何study.Conclusions的第一和第二半之间的除颤成功率的差异-Langendorff兔心脏模型是适合于评估电纤维性颤动和除颤机制。当使用双相脉冲而不是双相脉冲时，可以用较低的能量实现除颤。
Study objective - The aims were to develop a Langendorff rabbit heart model and to compare monophasic and biphasic defibrillation pulses.Design - Hearts were perfused with a Krebs-Henseleit solution and two 1.4 cm2 Pt-Ir mesh patch electrodes were sutured onto the ventricles. A 5 ms monophasic or 10 ms biphasic pulse, with randomly selected voltages of 30, 50, 70, 90, 110, or 130 V, defibrillated the heart after 10 s of fibrillation.Subjects - 11 adult male New Zealand white rabbits weighing 2.8(0.27) kg, were used for the studies.Measurements and main results - A total of 72 fibrillation and defibrillation sequences were conducted in each preparation. The results were fitted to a sigmoidal dose-response curve by logistic regression analysis. Voltage and energy values from the fitted data at 50% and 80% success (V50, V80, E50, E80) indicated a significantly lower (p < 0.05) defibrillation threshold voltage and energy for the biphasic waveform [V50 = 48 (SD19) V, V80 = 87(27) V, E50 = 0.15(0.12) J, E80 = 0.48(0.29) J] compared with the monophasic waveform [V50 = 79(20) V, V80 = 110(20) V, E50 = 0.27(0.12) J, E80 = 0.5(0.12) J]. There was no observed difference in defibrillation success rate between the first and second halves of any study.Conclusions - The Langendorff rabbit heart model is suitable for assessing electrical fibrillation and defibrillation mechanisms. Defibrillation can be achieved with a lower energy when using a biphasic rather than a monophasic pulse.