Screening for Antitumor Activities of Endophytic Fungi Isolated from Four Species of Gymnoperm
Screening for Antitumor Activities of Endophytic Fungi Isolated from Four Species of Gymnoperm
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发表时间：
2002
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通讯作者：
Huang Yao-jian
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作者：
Huang Yao-jian
从巨叶豆杉、红豆杉、红豆杉和银杏中分离得到112株内生真菌。为获得抗肿瘤活性较高的菌株，以盐蒿模型为预筛选方法，鉴定出39株（ld508）为高活性真菌（占总分离菌株的34.8%）。以MTT法为再筛选方法，9株内生真菌（占分离菌株总数的8.0%）可寄生于KB或HL-60细胞（ID 5080）的生长。巨叶豆杉内生真菌具有细胞毒活性的比例分别为7.4%、11.1%和8.3%。结果表明，与药用植物共生的内生真菌在抗肿瘤生物活性物质的研究和开发中具有重要的作用。
One hundred and twelve strains of endophytic fungi were isolated from Podocarpus macrophyllus, Taxus wallichiana ver mairei,Cephalotaxus fortunei and Ginkgo bilobal. For getting the strains with high activity of anti-tumor, with Artemia Salina model as the pre-screening method, thirty nine strains (LD 508) were identified as highly active fungi (34.8% of total isolated strains). With MTT assay as re-screening approach ,the results show that nine strains of endophytic fungi (8.0% of the total isolated stains) can inhabit the growth of KB or HL-60 cell (ID 5080). 7.4% of endophytic fungi isolated from Podocarpus macrophyllus, 11.1% from Taxus wallichiana ver mairei and 8.3% from Cephalotaxus fortunei have cytotoxic activities, respectively. The results indicate that endophytic fungi accreting with pharmaceutical plants have significant functions in research and development of the anti-tumor bioactive materials.