Organic Solvent-Free Preparation of Chitosan Nanofibers with High Specific Surface Charge and Their Application in Biomaterials
Organic Solvent-Free Preparation of Chitosan Nanofibers with High Specific Surface Charge and Their Application in Biomaterials
复制标题
高比表面电荷壳聚糖纳米纤维的无有机溶剂制备及其在生物材料中的应用
DOI：
10.1021/acsami.0c21796
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发表时间：
2021-02-24
影响因子：
9.5
通讯作者：
Li, Xiaofeng
中科院分区：
文献类型：
--
作者：
Zhang, Sihan;Zhao, Guanglei;Li, Xiaofeng
壳聚糖纳米纤维在生物组织工程材料中的应用已引起广泛关注。提出了一种无溶剂条件下制备直径为40-250 nm的根状壳聚糖纳米纤维的新方法。该方法包括溶胀-打浆-离心或溶胀-打浆-均质三步机械处理。所得的纳米纤维表现出高收率（>95%）和正的比表面电荷（高达+375 mu eq/g），并且可以均匀地分散在水相中。CSNFs独特的纤维形状和良好的长径比赋予了壳聚糖滤纸（CSNFP）制品优异的力学性能，其湿拉伸强度是普通壳聚糖膜的近5倍。此外，颅骨来源的前成骨细胞表现出更高的粘附效率和增殖CSNFP比壳聚糖膜。壳聚糖支架产品也有利于前成骨细胞的附着，并允许它们在三维空间中生长。该方法对壳聚糖纳米纤维的工业化具有重要的工业潜力，壳聚糖纳米纤维可能在各种生物材料中具有广泛的应用。
The application of chitosan nanofibers in biological tissue-engineering materials has attracted wide attention. A novel and organic solvent-free method was developed for the fabrication of rootlike chitosan nanofibers (CSNFs) with diameters of 40-250 nm. This method includes three-step mechanical processing of swelling-beating-centrifugation or swelling-beating-homogenization. The obtained nanofibers showed high yields (>95%) and positive specific surface charges (up to +375 mu eq/g) and could be uniformly dispersed in the aqueous phase. The unique fiber shape and the good length-to-diameter ratio of CSNFs endowed chitosan nanofiber paper (CSNFP) products with excellent mechanical properties, and the wet tensile strength of the CSNFPs was nearly five times higher than common chitosan films. In addition, the calvaria-derived preosteoblastic cells exhibited a higher adherence efficiency and proliferation on CSNFP than on chitosan films. The chitosan nanofiber scaffold products also benefited the attachment of preosteoblastic cells and allowed them to grow in three dimensions. This method has significant industrial potential for the industrialization of chitosan nanofibers, which may have broad applications in various biomaterials.