Detection of ovomucoid- specific low-affinity IgE in infants and its relationship to eczema.
Detection of ovomucoid- specific low-affinity IgE in infants and its relationship to eczema.
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婴儿卵类粘蛋白特异性低亲和力 IgE 的检测及其与湿疹的关系。
DOI：
10.1111/pai.12702
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Fukao T.
中科院分区：
文献类型：
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作者：
Kawamoto N;Kamemura N;Kido H;Fukao T.
过敏原特异性低亲和力IgE以前通过高灵敏的密集羧化蛋白(DCP)芯片在脐带血中检测到，但不能通过免疫CAP检测到。在这里，我们调查了婴儿早期低亲和力IgE的存在，并观察了它与湿疹的关系。方法我们进行了出生队列研究，收集出生时、6个月和14个月大的血清(n=110)。我们用免疫CAP或DCP芯片检测卵粘蛋白(OM)和蛋清(EW)特异性的IgE(SIgE)，并在有或没有温和的变色剂二乙胺(DEA)的情况下用结合抑制试验分析sIgE的抗原亲和力。结果DCP芯片检测的OM-sIgE与免疫CAP法检测的OM-sIgE显著相关，但部分经DCP芯片检测为OM-sIgE阳性的标本免疫CAP检测为OM-sIgE阴性。对14个月时DCP芯片判定为OM-sIgE阳性的受试者进行DEA治疗后的结合抑制分析，免疫CAP检测OM-和EW-sIgE在6个月和14个月时为阴性组，DEA治疗后结合抑制曲线移动幅度大于这些时间段的阳性者，表明14个月时存在低亲和力sIgE抗体。Logistic回归分析发现，6~14个月的持续性湿疹是发生高亲和力而非低亲和力sIgE的重要危险因素。结论婴幼儿外周血中含有过敏原特异性低亲和力sIgE。持续性湿疹与高亲和力的IgE有关，但与低亲和力的IgE无关。
BackgroundAllergen‐specific low‐affinity IgE was previously detected in cord blood by a highly sensitive densely carboxylated protein (DCP) chip, but not by ImmunoCAP. Here, we investigated the presence of low‐affinity IgE during the early life of infants and observed its relationship with eczema.MethodsWe conducted a birth cohort study, collecting sera at birth and 6 and 14 months of age (n = 110). We monitored the ovomucoid (OM)‐ and egg white (EW)‐specific IgE (sIgE) by ImmunoCAP or DCP chip and analyzed the antigen affinity of sIgE by binding inhibition assays in the presence or absence of a mild chaotropic agent, diethyl amine (DEA). The low‐ and high‐affinity OM‐sIgEs and sensitization risk factors were analyzed by a multivariate logistic analysis.ResultsThe OM‐sIgE measured by DCP chip significantly correlated with that measured by ImmunoCAP, but some samples assessed as OM‐sIgE positive by DCP chip were considered OM‐sIgE negative by ImmunoCAP. Binding inhibition analysis after DEA treatment was performed for participants judged as OM‐sIgE positive by DCP chip at 14 M. The group assessed as negative for OM‐ and EW‐sIgE by ImmunoCAP at 6 and 14 months showed a larger binding inhibition curve shift after DEA treatment than did the group assessed as positive at these times, indicating the presence of low‐affinity sIgE antibodies at 14 months. The logistic regression analysis found that persistent eczema from 6 to 14 months is a significant risk factor for developing high‐affinity, but not low‐affinity, sIgE.ConclusionsHuman infant peripheral blood contains allergen‐specific low‐affinity sIgE. Persistent eczema is related to the development of high‐affinity, but not low‐affinity, IgE.