Enhanced compatibility of chemically modified titanium surface with periodontal ligament cells.
Enhanced compatibility of chemically modified titanium surface with periodontal ligament cells.
复制标题
化学改性钛表面与牙周膜细胞的相容性增强。
DOI：
10.1016/j.apsusc.2012.07.091
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发表时间：
2012
影响因子：
6.7
通讯作者：
Takashi Kado
中科院分区：
文献类型：
--
作者：
Jun Yokota;Naoyuki Chosa;Shunsuke Sawada;Naoto Okubo;Noriko Takahashi;Tomokazu Hasegawa;Hisatomo Kondo;Akira Ishisaki;Takashi Kado
为了改善钛表面与人牙周韧带细胞(HPDLCs)的相容性，开发了一种简单的化学改性方法，将细胞黏附分子固定在钛表面。抛光后的钛盘在1%(v/v)对乙烯基苯甲酸溶液中浸泡2h，在表面引入羧基。钛片用去离子蒸馏水清洗后，浸泡在含0.1 mg/mlGly-Arg-Gly-Asp-Ser(GRGDS)、人血浆纤维连接蛋白(PFN)或小牛皮I型胶原(Col)的1.47%1-乙基-3-(3-二甲氨基丙基)碳二亚胺溶液中，通过多肽键的形成将细胞黏附分子共价固定在钛表面。在扫描探针显微镜下，钴固定化的钛表面表现出比抛光的钛表面更高的纳米粗糙度。在PFN和COL固定的钛表面上附着的HPDLC的数量是抛光的钛表面的两倍。细胞较大，细胞突起在PFN和Col固定的钛表面比在抛光的钛表面伸展的程度更大(p<；n0.05)。与抛光前、GRGDS固定化和PFN固定化的钛表面相比，钴固定的钛表面HPDLCs在细胞突起尖端和细胞轮廓上有更广泛的纽蛋白表达。结论：细胞黏附分子成功地固定在钛表面，改善了表面与HPDLCs的相容性。固定化的钛表面可用于形成钛种植体周围的韧带样组织。
A simple chemical modification method was developed to immobilize cell-adhesive molecules on a titanium surface to improve its compatibility with human periodontal ligament cells (HPDLCs).The polished titanium disk was immersed in 1% (v/v) p-vinylbenzoic acid solution for 2 h to introduce carboxyl groups onto the surface. After rinsing with distilled deionized water, the titanium disk was dipped into 1.47% 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide solution containing 0.1 mg/ml Gly-Arg-Gly-Asp-Ser (GRGDS), human plasma fibronectin (pFN), or type I collagen from calf skin (Col) to covalently immobilize the cell-adhesive molecules on the titanium surface via formation of peptide bonds.X-ray photoelectron spectroscopy analyses revealed that cell-adhesive molecules were successfully immobilized on the titanium surfaces. The Col-immobilized titanium surface revealed higher values regarding nano rough characteristics than the as-polished titanium surface under scanning probe microscopy. The number of HPDLCs attached to both the pFN- and Col-immobilized titanium surfaces was twice that attached to the as-polished titanium surfaces. The cells were larger with the cellular processes that stretched to a greater extent on the pFN- and Col-immobilized titanium surfaces than on the as-polished titanium surface (p< 0.05). HPDLCs on the Col-immobilized titanium surfaces showed more extensive expression of vinculin at the tips of cell projections and more contiguously along the cell outline than on the as-polished, GRGDS-immobilized and pFN-immobilized titanium surfaces.It was concluded that cell-adhesive molecules successfully immobilized on the titanium surface and improved the compatibility of the surface with HPDLCs. The Col-immobilized titanium surface could be used for forming ligament-like tissues around titanium dental implants.