Effect of RFRP-3 on reproduction is sex- and developmental status-dependent in the striped hamster (Cricetulus barabensis).
Effect of RFRP-3 on reproduction is sex- and developmental status-dependent in the striped hamster (Cricetulus barabensis).
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DOI：
10.1016/j.gene.2014.06.054
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发表时间：
2014-09
期刊：
影响因子：
3.5
通讯作者：
Lei Zhao;Min Zhong;Huiliang Xue;Jinghua Ding;Shuo Wang;Jin-hui Xu;Lei Chen;Laixiang Xu
中科院分区：
文献类型：
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作者：
Lei Zhao;Min Zhong;Huiliang Xue;Jinghua Ding;Shuo Wang;Jin-hui Xu;Lei Chen;Laixiang Xu
RFamide相关肽（RFRPs）与抑制促性腺激素释放的促性腺激素抑制激素（GnIH）直接相关。在啮齿类动物中只有两个RFRP序列（RFRP-1和RFRP-3）编码。RFRP-3被认为是GnRH的假想抑制剂，在短时间内对雄性叙利亚仓鼠和雄性西伯利亚仓鼠显示出刺激作用。黑线仓鼠（Cricetulus barabensis）是我国北方农田的优势害鼠，具有较高的繁殖能力，可作为研究其繁殖机制的模式动物。然而，RFRP-3对条纹仓鼠生殖活动的影响还不太清楚。本研究从黑线仓鼠下丘脑中克隆了643 bp的RFRP cDNA，其ORF为570 bp，编码两个RFRP序列：SPAPANKVPHSAANLPLRF-NH 2（C. barabensisRFRP-1）和TLSRVPSLPQRF-NH 2（C. barabensisRFRP-3）。本研究还采用RT-PCR方法检测了不同发育阶段（7周龄、13周龄和1.5岁）的金黄地鼠下丘脑RFRP mRNA和GnRHmRNA的表达变化，其中13周龄雌性个体处于发情期。7周龄和1.5岁的条纹仓鼠是非繁殖个体，13周龄的仓鼠有繁殖现象。最高的下丘脑RFRPmRNA水平被发现在繁殖男性相比，非繁殖男性。相反，最低的RFRPmRNA水平在下丘脑中观察到繁殖的女性，没有显着水平时，繁殖的女性相比，7周龄的个体。此外，GnRH表达水平的调查显示，在整个发育阶段（7周龄，13周龄和1.5岁）的表达下降的趋势，在两种性别。结果表明，RFRP-3在发情期雌性（r=-0.997，P = 0.035）和发情期雄性（r= 0.998，P = 0.029）黑线仓鼠体内分别存在显著的负相关和正相关，表明RFRP-3对雌性和雄性成年个体分别具有抑制和刺激作用。我们的研究结果表明，RFRP-3对生殖的影响是性别和发育状态依赖的条纹仓鼠。
RFamide-related peptides (RFRPs) are orthologous to gonadotropin-inhibitory hormone (GnIH) inhibiting gonadotropin release. There are only two RFRP sequences (RFRP-1 and RFRP-3) encoded in rodents. RFRP-3, which was considered as a hypothetical inhibitor on GnRH, shows a stimulatory effect on the male Syrian and male Siberian hamster in short days. As a dominant rodent pest in northern China farmland, the striped hamster (Cricetulus barabensis) has higher reproductive activities and could act as a model to study the mechanism of reproduction. However, the effect of RFRP-3 on the reproductive activity for the striped hamster is less understood. In the study, we cloned 643 bpRFRPcDNA from the striped hamster hypothalamus, which contained an ORF of 570 bp encoding two RFamide-related peptide (RFRP) sequences: SPAPANKVPHSAANLPLRF-NH2(C. barabensisRFRP-1) and TLSRVPSLPQRF-NH2(C. barabensisRFRP-3). We also investigated the expression variation ofRFRPmRNA andGnRHmRNA in the hypothalamus from hamsters with different developmental statuses (7-week-, 13-week- and 1.5-year-olds) using FQ-PCR, in which the 13-week-old female individuals were in estrous. The striped hamsters that are 7 weeks and 1.5 years old are non-breeding individuals, and those that are 13-week hamsters have breeding phenomena. The highest hypothalamusRFRPmRNA level was found in breeding males as compared to non-breeding males. Conversely, the lowestRFRPmRNA level in the hypothalamus was observed in breeding females, with no significant level when the breeding females were compared to the 7-week-old individuals. Additionally, the investigation ofGnRHexpression level showed a declining expression trend across the developmental stages (7-week-, 13-week- and 1.5-year-olds) in both sexes. Significant negative and positive relationships were detected in the 13-week estrous female (r= − 0.997,P= 0.035) and the 13-week male (r= 0.998,P= 0.029) striped hamsters respectively, which suggest that RFRP-3 has inhibitory and stimulatory effects on female and male adults respectively. Our results suggest that the effects of RFRP-3 on reproduction are sex- and developmental status-dependent in the striped hamster.