Cyclic phosphatidic acid influences the expression and regulation of cyclic nucleotide phosphodiesterase 3B and lipolysis in 3T3-L1 cells
Cyclic phosphatidic acid influences the expression and regulation of cyclic nucleotide phosphodiesterase 3B and lipolysis in 3T3-L1 cells
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DOI：
10.1016/j.bbrc.2010.11.076
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发表时间：
2011-01-07
影响因子：
3.1
通讯作者：
Murakami-Murofushi, Kimiko
中科院分区：
文献类型：
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作者：
Tsukahara, Tamotsu;Hanazawa, Shuwa;Murakami-Murofushi, Kimiko
环磷脂酸(CPA)广泛存在于从黏菌到人类的各种细胞中，其功能未知。我们先前报道，CPA通过抑制PPAR-γ的激活而显著抑制3T3-L1脂肪细胞的脂质积聚。在此，我们发现CPA降低了3T3-L1脂肪细胞内甘油三酯水平并抑制了磷酸二酯酶3B(PDE3B)的表达。PPAR在成脂过程中的激活可以被CPA阻断，然后通过抑制PDE3B的表达参与脂肪细胞的功能。我们还发现CPA作用后3T3-L1脂肪细胞内cAMP水平升高。这些发现有助于CPA参与3T3-L1脂肪细胞的脂解活性。我们的研究表明，CPA可能是一种治疗肥胖和肥胖相关疾病的化合物。(C)2010 Elsevier Inc.保留所有权利。
Cyclic phosphatidic acid (CPA) is found in cells from slime mold to humans and has a largely unknown function. We previously reported that cPA significantly inhibited the lipid accumulation in 3T3-L1 adipocytes through inhibition of PPAR gamma activation. We find here that CPA reduced intracellular triglyceride levels and inhibited the phosphodiesterase 3B (PDE3B) expression in 3T3-L1 adipocytes. PPAR gamma activation in adipogenesis that can be blocked by treatment with cPA then participates in adipocyte function through inhibition of PDE3B expression. We also found the intracellular cAMP levels in 3T3-L1 adipocytes increased after exposure to cPA. These findings contribute to the participation of cPA on the lipolytic activity in 3T3-L1 adipocytes. Our studies imply that CPA might be a therapeutic compound in the treatment of obesity and obesity-related diseases. (C) 2010 Elsevier Inc. All rights reserved.