The roles of Golgi and endoplasmic reticulum in the synthesis and assembly of lipoprotein lipids in rat hepatocytes.
The roles of Golgi and endoplasmic reticulum in the synthesis and assembly of lipoprotein lipids in rat hepatocytes.
复制标题
高尔基体和内质网在大鼠肝细胞脂蛋白脂质合成和组装中的作用。
作者：
J. Higgins;J. Hutson
从大鼠肝脏中分离出来自内质网和高尔基体组分的微生物体，并通过碳酸钠处理或通过法国压力池分离出内容物和膜亚组分。微粒体内含有颗粒，甘油三酯的摩尔浓度比磷脂、胆固醇和胆固醇酯大10倍。相比之下，高尔基体中含有的颗粒大约大50%，而甘油三酯的含量相对较少。[~3H]棕榈酸最初被结合到甘油三酯池中，与微粒体膜结合，随后被隔离在脑池空间，然后转移到高尔基体区域。[~3H]棕榈酸与磷脂的结合速度较慢。微粒体中磷脂的比活性约为膜的两倍，表明新合成的磷脂优先被隔离。而高尔基体膜的比活力和磷脂含量与微粒体膜相似。这些观察表明，要分泌的甘油三酯与少量其他脂质一起被隔离在内质网的脑池空间中。当富含甘油三酯的颗粒到达高尔基池时，大量的磷脂和胆固醇显然会被添加到这些颗粒中。
Microsomes, derived from the endoplasmic reticulum and Golgi fractions, were isolated from rat liver and separated into content and membrane subfractions by treatment with sodium carbonate or by passage through the French pressure cell. The microsomal content contained particles, which had a triglyceride molar concentration tenfold greater than that of phospholipid, cholesterol, and cholesteryl ester. In contrast, the Golgi content contained particles approximately 50% larger which contained proportionally less triglyceride. [3H]palmitic acid was initially incorporated into a pool of triglyceride, associated with the microsomal membrane, subsequently sequestered in the cisternal space, and then transferred to the Golgi region. [3H]palmitic acid was incorporated more slowly into phospholipid. The specific activity of the microsomal content phospholipid reached a level approximately twice that of the membrane, suggesting that newly synthesized phospholipid is preferentially sequestered. However, the specific activities of the Golgi membrane and content phospholipid were similar to that of the microsomal membrane. These observations suggest that the triglyceride to be secreted is sequestered into the cisternal space of the endoplasmic reticulum together with only small amounts of other lipids. The bulk of the phospholipid and cholesterol to be secreted is apparently added to the triglyceride-rich particles when these reach the Golgi cisternae.
培养的大鼠肝细胞合成和分泌极低密度、低密度和高密度脂蛋白的时间相关变化。
DOI：
10.1016/0005-2760(81)90196-x
发表时间：
1981
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Bell-Quint,J;Forte,T
通讯作者：
Forte,T