The recent ancestry of Middle East respiratory syndrome coronavirus in Korea has been shaped by recombination.
The recent ancestry of Middle East respiratory syndrome coronavirus in Korea has been shaped by recombination.
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DOI：
10.1038/srep18825
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发表时间：
2016-01-06
影响因子：
4.6
通讯作者：
Park MS
中科院分区：
文献类型：
--
作者：
Kim JI;Kim YJ;Lemey P;Lee I;Park S;Bae JY;Kim D;Kim H;Jang SI;Yang JS;Kim H;Kim DW;Nam JG;Kim SS;Kim K;Myun Lee J;Song MK;Song D;Chang J;Hong KJ;Bae YS;Song JW;Lee JS;Park MS
中东呼吸综合征冠状病毒（MERS-CoV）导致严重的人类呼吸道疾病病例。自2012年以来，受害者主要来自中东国家，或零星来自访问中东的旅行者播种的其他一些地理区域。这种通过旅行的引入导致了2015年5月在韩国爆发的MERS冠状病毒疫情，在不到一个月的时间里造成了140多例确诊病例。利用70个MERS冠状病毒分离株的全基因组序列，包括韩国和中国分离株的最新序列，我们重建了完整基因组和单个蛋白编码区的系统发育关系。韩国MERS-CoV毒株与2015年采样的两个沙特阿拉伯和一个中国毒株一起聚集在先前建立的Hafr-Al-Batin-1_2013分支中。虽然这四个菌株在整个蛋白质编码区保持单系性，但该分支在整个基因组中显示出不同的系统发育关系，表明与先前报道的推定重组菌株不同的共享独特的重组模式。我们的研究结果表明，最近的祖先韩国及其相关的MERS冠状病毒株的特点是独特的镶嵌基因组模式，是不同于其他推定的重组。
Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe cases of human respiratory disease. Since 2012, the victims have mainly come from the Middle East countries or sporadically from some other geographical regions seeded by the travelers who visited the Middle East. Such an introduction through travelling led to the emergence of a MERS-CoV outbreak in Korea in May 2015, which caused more than 140 confirmed human cases in less than a month. Using 70 complete genome sequences of MERS-CoV isolates, including the most recent sequences for the Korean and Chinese isolates, we reconstructed the phylogenetic relationships of the complete genome and the individual protein coding regions. The Korean MERS-CoV strain clustered in the previously established Hafr-Al-Batin-1_2013 clade together with two Saudi Arabian and one Chinese strain sampled in 2015. Although these four strains remained monophyletic in the entire protein-coding region, this clade showed different phylogenetic relationships across the genome, indicating a shared unique recombination pattern that is different from previously reported putative recombination strains. Our findings suggest that the recent ancestor of the Korean and its related MERS-CoV strains is characterized by unique mosaic genome pattern that is different from other putative recombinants.