Identification and molecular characterization of an efflux system involved in Pseudomonas putida S12 multidrug resistance
Identification and molecular characterization of an efflux system involved in Pseudomonas putida S12 multidrug resistance
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DOI：
10.1099/00221287-147-1-43
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发表时间：
2001-01-01
期刊：
影响因子：
2.8
通讯作者：
de Bont, JAM
中科院分区：
文献类型：
--
作者：
Kieboom, J;de Bont, JAM
作者之前描述了 srpABC，这是一种参与耐溶剂恶臭假单胞菌 512 中质子依赖性溶剂流出的操纵子。最近，研究表明有机溶剂而非抗生素诱导该操纵子。在本研究中，作者基于两个分离的氯霉素敏感转座子突变体描述了一种新的外排泵，命名为 ArpABC。 ArpABC操纵子参与多种抗生素的主动外流，例如四环素、氯霉素。羧苄西林、链霉素。红霉素和新生霉素。由所涉及的三个基因编码的推导的氨基酸序列显示出与抗性/结瘤/细胞分裂家族的蛋白质惊人的相似性，这些蛋白质参与有机溶剂和多种药物流出。这些发现表明，ArpABC 与有机溶剂和多种抗生素的 MepABC 和 TtgABC 外排系统高度同源。然而，ArpABC 对恶臭假单胞菌 512 的有机溶剂耐受性没有贡献，而仅参与多药耐药性。
The authors previously described srpABC, an operon involved in proton-dependent solvent efflux in the solvent-tolerant Pseudomonas putida 512. Recently, it was shown that organic solvents and not antibiotics induce this operon. In the present study, the authors characterize a new efflux pump, designated ArpABC, on the basis of two isolated chloramphenicol-sensitive transposon mutants. The ArpABC operon is involved in the active efflux of multiple antibiotics, such as tetracycline, chloramphenicol. carbenicillin, streptomycin. erythromycin and novobiocin. The deduced amino acid sequences encoded by the three genes involved show a striking resemblance to proteins of the resistance/nodulation/cell division family, which are involved in both organic solvent and multiple drug efflux. These findings demonstrate that ArpABC is highly homologous to the MepABC and TtgABC efflux systems for organic solvents and multiple antibiotics. However, ArpABC does not contribute to organic solvent tolerance in P. putida 512 but is solely involved in multidrug resistance.