Coccolithovirus (Phycodnaviridae):: Characterisation of a new large dsDNA algal virus that infects Emiliania huxleyi
Coccolithovirus (Phycodnaviridae):: Characterisation of a new large dsDNA algal virus that infects Emiliania huxleyi
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DOI：
10.1007/s00705-002-0841-3
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发表时间：
2002-09-01
影响因子：
2.7
通讯作者：
Wilson, WH
中科院分区：
文献类型：
--
作者：
Schroeder, DC;Oke, J;Wilson, WH
从E. 1999年7月和2001年7月/8月，huxleyi在英国普利茅斯海岸附近开花，来自E. 2000年6月，在挪威卑尔根（卑尔根）附近峡湾进行的围隔实验中，诱导了赫胥黎水华。透射电子显微镜显示，所有10个病毒分离物的直径为170-200 nm，具有二十面体对称性。它们的密度在CsCl梯度中约为1.2，并且它们具有大小约为410 kb的大双链DNA基因组。这些病毒的DNA聚合酶基因的系统发育分析表明，EhV属于一个新的属藻类病毒，藻DNA病毒科。我们建议将这种新病毒命名为球石病毒属。通过病毒分离株的宿主范围分析和编码其推定的主要衣壳蛋白的基因片段的序列分析，阐明了球石病毒成员内的差异。新属的10个病毒分离物均只感染E. huxleyi菌株（CCMP 1516、CCMP 374和L）表现出低的二甲基磺基丙酸裂解酶（DMSP-裂解酶）活性，而E.具有高的DMSP裂解酶活性的huxleyi菌株（CCMP 373和CCMP 379）对感染具有抗性。
Emiliania huxleyi-specific viruses (EhV) were isolated from E. huxleyi blooms off the coast of Plymouth, UK, in July 1999 and July/August 2001, and from an E. huxleyi bloom induced during a mesocosm experiment in a fjord off Bergen, Norway, during June 2000. Transmission electron microscopy revealed that all 10 virus isolates are 170-200 nm in diameter with an icosahedral symmetry. Their density is approximately 1.2 in CsCl gradients and they have large double stranded DNA genomes approximately 410 kb in size. Phylogenetic analysis of the DNA polymerase genes of these viruses suggests that EhV belongs to a new genus within the family of algal viruses, Phycodnaviridae. We propose to name this new virus genus Coccolithovirus. Differences within members of the Coccolithovirus were elucidated by host range analysis of the virus isolates and sequence analysis of a gene fragment encoding part of their putative major capsid protein. All 10 virus isolates within this new genus only infected E. huxleyi strains that have previously been shown to exhibit low dimethylsulphoniopropionate lyase (DMSP-lyase) activity (CCMP1516, CCMP374 and L), while E. huxleyi strains with high DMSP-lyase activity (CCMP373 and CCMP379) were resistant to infection.