Shifts in the pelagic ammonia-oxidizing microbial communities along the eutrophic estuary of Yong River in Ningbo City, China.
Shifts in the pelagic ammonia-oxidizing microbial communities along the eutrophic estuary of Yong River in Ningbo City, China.
复制标题
中国宁波市甬江富营养化河口沿岸中上层氨氧化微生物群落的变化。
DOI：
10.3389/fmicb.2015.01180
复制
发表时间：
2015
影响因子：
5.2
通讯作者：
Laanbroek HJ
中科院分区：
文献类型：
--
作者：
Zhang Q;Tang F;Zhou Y;Xu J;Chen H;Wang M;Laanbroek HJ
好氧氨氧化在氮循环中起着关键作用，负责微生物的多样性受环境因素的调节。调查了中国东部宁波永河环境梯度沿线表层水体中氨氧化古菌和氨氧化细菌的丰度和组成。水样采集自宁波市区运河淡水、永江下游微咸水和杭州湾近岸海水。氨氧化微生物的活性和多样性的变化与环境因素的变化同步发生，其中盐度以及氨和氧的有效性。AOA丰度始终高于AOB，且与氨氧化活性有关。微咸水和海水中AOA/AOB的比值显著高于淡水。微咸水和海水的AOA和AOB具有相似的群落组成，但它们与城市内陆淡水的相似性分别只有31%和35%。来自淡水的AOA-Amoa序列大多与陆地环境中获得的序列有关，而从微咸水和沿海地区采集的AOA-Amoa序列在海洋、海岸和陆地生态系统中普遍存在。所有来自淡水的AOB都属于亚硝酸单胞菌，来自微咸水和海水的AOB主要属于亚硝酸螺旋体。
Aerobic ammonia oxidation plays a key role in the nitrogen cycle, and the diversity of the responsible microorganisms is regulated by environmental factors. Abundance and composition of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) were investigated in the surface waters along an environmental gradient of the Yong River in Ningbo, East China. Water samples were collected from three pelagic zones: (1) freshwaters in the urban canals of Ningbo, (2) brackish waters in the downstream Yong River, and (3) coastal marine water of Hangzhou Bay. Shifts in activity and diversity of the ammonia-oxidizing microorganisms occurred simultaneously with changes in environmental factors, among which salinity and the availabilities of ammonium and oxygen. The AOA abundance was always higher than that of AOB and was related to the ammonia oxidation activity. The ratios of AOA/AOB in the brackish and marine waters were significantly higher than those found in freshwaters. Both AOA and AOB showed similar community compositions in brackish and marine waters, but only 31 and 35% similarity, respectively, between these waters and the urban inland freshwaters. Most of AOA-amoA sequences from freshwater were affiliated with sequences obtained from terrestrial environments and those collected from brackish and coastal areas were ubiquitous in marine, coastal, and terrestrial ecosystems. All AOB from freshwaters belonged to Nitrosomonas, and the AOB from brackish and marine waters mainly belonged to Nitrosospira.