A fluorescent probe capable of discriminately and simultaneously detecting dl-dithiothreitol and single sulfhydryl-containing thiols
A fluorescent probe capable of discriminately and simultaneously detecting dl-dithiothreitol and single sulfhydryl-containing thiols
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DOI：
10.1016/j.snb.2015.10.036
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发表时间：
2016-03
影响因子：
8.4
通讯作者：
Sihua Guo;Fangyuan Zheng;Fang Zeng;Shuizhu Wu
中科院分区：
文献类型：
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作者：
Sihua Guo;Fangyuan Zheng;Fang Zeng;Shuizhu Wu
dl-二硫苏糖醇（DTT）主要用于临床和实验室领域。它还参与多种细胞机制，可用于治疗某些疾病，例如胱氨酸病或由离子或金属毒性引起的医疗状况。因此，生物环境中的DTT检测具有重要意义。然而，目前大多数荧光检测系统无法区分 DTT 和单一含巯基的生物硫醇。在此，通过将马来酰亚胺与芘荧光团连接，合成了 DTT 荧光探针。它对不同发射带的 DTT 或单个含巯基硫醇做出响应，使其能够从具有单个单键 SH 的硫醇中区分检测 DTT。该探针对二硫苏糖醇快速响应，并通过芘部分的准分子发射显示出高选择性。至于探针的荧光强度与 DTT 浓度的关系，可以观察到两个线性范围（0-3 和 4.5-6.0 μM），DTT 的检测限为 0.07 μM。此外，该探针具有低细胞毒性，可成功用于 L929 和 Hela 细胞系的荧光成像。
dl-Dithiothreitol (DTT) is used mainly in clinical and laboratorial fields. It also participates in several cellular mechanisms and can be used in some diseases treatment like cystinosis or medical conditions resulting from ion or metal toxicity. Hence, DTT detection in biological milieu is of great significance. However, most of the current fluorescent detection systems cannot discriminate DTT from single sulfhydryl-containing biothiols. Herein, a fluorescent probe for DTT has been synthesized by linking maleimide to a pyrene fluorophore. It responds to DTT or single sulfhydryl-containing thiols at different emission bands, affording it the capability to discriminately detect DTT from those thiols with single single bondSH. The probe responds to dithiothreitol quickly and shows high selectivity through excimer emission of pyrene moieties. As for the fluorescence intensity of the probe versus DTT concentration, two linear ranges (0–3 and 4.5–6.0 μM) can be observed, and the detection limit for DTT is 0.07 μM. Furthermore, the probe exhibits low cytotoxicity and can be successfully used in fluorescence imaging in both L929 and Hela cell lines.