APPARENT SOURCES OF THE A GENOMES OF WHEATS INFERRED FROM POLYMORPHISM IN ABUNDANCE AND RESTRICTION FRAGMENT LENGTH OF REPEATED NUCLEOTIDE-SEQUENCES
APPARENT SOURCES OF THE A GENOMES OF WHEATS INFERRED FROM POLYMORPHISM IN ABUNDANCE AND RESTRICTION FRAGMENT LENGTH OF REPEATED NUCLEOTIDE-SEQUENCES
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DOI：
10.1139/g88-115
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发表时间：
1988-10-01
期刊：
影响因子：
3.1
通讯作者：
CASSIDY, B
中科院分区：
文献类型：
--
作者：
DVORAK, J;MCGUIRE, PE;CASSIDY, B
以野生二倍体小麦一粒小麦（Triticummonococcumssp.）Aegilopoides（syn. T. baeoticum）筛选重复核苷酸序列的克隆。分离到7个DNA片段，它们在二倍体T.单球孢属Aegilopoides（基因组A）的基因组比二倍体Speltoides小麦（基因组B）的基因组中的基因组高。然后用这些克隆来确定两个野生二倍体小麦中的哪一个，T。M. SSP. aegilopoides或T. urartu是二倍体小麦T. M. SSP. monococcum、野生四倍体小麦T. M. SSP. Aegilopoides of T. urartu是二倍体小麦T. M. SSP. monococcum、野生和六倍体面包T.小麦7个重复序列中有3个可区分T m基因组。SSP. aegilopoides与T.在重复序列丰度、限制性片段长度多态性、或两者上都有优势。在T. mi. SSP. Aegilopoides和多倍体小麦的A基因组。由此得出结论，T. M. SSP.驯化的单球孢菌为T. M. SSP. Aegilopoides的A染色体组与T.乌拉图。本文的结果表明，重复核苷酸序列的多态性在多倍体植物基因组起源的研究中具有实用价值。
Four hundred random DNA fragment clones of wild diploid wheat Triticum monococcum ssp. aegilopoides (syn. T. baeoticum) were screened for clones of repeated nucleotide sequences. Seven DNA fragments were isolated that were more abundant by one order of magnitude or more in the genome of diploid T. monococcum ssp. aegilopoides (genome A) than in the genome of diploid Triticum speltoides (genome Bs). These clones were then used to determine which of the two wild diploid wheats, T. m. ssp. aegilopoides or T. urartu, was the ancestor of domesticated diploid wheat T. m. ssp. monococcum, wild tetraploid wheats T. m. ssp. aegilopoides of T. urartu, was the ancestor of domesticated diploid wheat T. m. ssp. monococcum, wild and hexaploid bread T. aestivum. Three of the seven cloned repeated nucleotide sequences differentiated the genome o T m. ssp. aegilopoides from that of T. urartu in repeated sequence abundance, restriction fragment length polymorphism, or both. THe same distinctions were observed between the a genome of T. mi. ssp. aegilopoides and the A genomes of polyploid wheats. From this it was concluded that the species from which T. m. ssp. monococcum was domesticated was T. m. ssp. aegilopoides but that the A genomes of the polyploid wheats are equivalent to that of T. urartu. The results presented here demonstrate the utility of polymorphism in repeated nucleotide sequences in the investigation of the origin of genomes in polyploid plants.