Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD
Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD
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DOI：
10.1016/j.jpba.2006.01.058
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发表时间：
2006-08-28
影响因子：
3.4
通讯作者：
Cui, X. M.
中科院分区：
文献类型：
--
作者：
Wan, J. B.;Yang, F. Q.;Cui, X. M.
采用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）和加压液体萃取（PLE）测定了三七不同部位，包括根、纤维根、根茎、茎、叶、花和种子的化学特性。对三七不同部位的八种主要皂苷，即三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rb3和Rd进行了定量比较。色谱图显示，三七地下部分（根、须根、根茎）和地上部分（叶和花）之间存在显着差异，但地下部分（0.965+/-0.029，n=12）和地上部分（0.987+/-0.014，n=5）测试样品整个色谱图的相似性分别相似。特别是，在三七种子中未检测到皂苷。基于8个被调查的皂苷或人参皂苷Rg1/Rb1和人参皂苷Rb3/Rb1的含量比进行层次聚类分析表明，来自三七不同部位的样品分为三个主要聚类。一簇为地下部分，含有丰富的原人参三醇和原人参二醇型皂苷。叶与花同生，仅含原人参二醇型皂苷。尤其是地下部分稀有的人参皂苷Rc、Rb2和Rb3，在三七地上部分含量丰富。三七的茎是另一簇。基于聚类分析，揭示了三七不同部位的化学特征。它们分别是复合簇（地下部分）、原人参二醇簇（地上部分）和中间簇（茎），介于两个典型簇之间。结果表明，三七地下部分和地上部分的化学特性存在明显差异，有助于三七的药理评价和质量控制。 (c) 2006 Elsevier B.V. 保留所有权利。
The chemical characteristics for different parts of Panax notoginseng, including root, fibre root, rhizome, stem, leaf, flower and seed, were determined using high performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and pressurized liquid extraction (PLE). Eight major saponins, namely notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rb3 and Rd were also quantitatively compared among the different parts of P. notoginseng. The chromatograms showed that there was significant difference between underground (root, fibre root, rhizome) and aerial (leaf and flower) parts from P. notoginseng, though the similarities of entire chromatographic patterns among tested samples from underground (0.965 +/- 0.029, n = 12) and aerial parts (0.987 +/- 0.014, n = 5) were similar, respectively. Especially, no saponin was detected in the seed of R notoginseng. Hierarchical clustering analysis based on eight investigated saponins or the ratios of contents for ginsenoside Rg1/Rb1 and ginsenoside Rb3/Rb1 showed that the samples from different parts of P. notoginseng were divided into three main clusters. One cluster was underground parts, which contained rich protopanaxatriol and protopanaxadiol types saponins. The leaf and flower were in the same cluster, which contained protopanaxadiol type saponins only. Especially, ginsenoside Rc, Rb2 and Rb3, rare in the underground parts, were rich in aerial parts of P. notoginseng. The stem of P. notoginseng was another cluster. Based on the cluster analysis, the chemical characteristics for different parts of P notoginseng were revealed. They are composite cluster (underground parts), protopanaxadiol cluster (aerial parts) and interim (stem) cluster, which was the one between the two typical clusters, respectively. The result shows that chemical characteristics of underground parts and aerial parts from R notoginseng are obviously different, which is helpful for pharmacological evaluation and quality control of P. notoginseng. (c) 2006 Elsevier B.V. All rights reserved.