EOSINOPHIL MIGRATION IN ATOPIC DERMATITIS-I - INCREASED MIGRATORY RESPONSES TO N-FORMYL-METHIONYL-LEUCYL-PHENYLALANINE, NEUTROPHIL-ACTIVATING FACTOR, PLATELET-ACTIVATING-FACTOR, AND PLATELET FACTOR-IV
EOSINOPHIL MIGRATION IN ATOPIC DERMATITIS-I - INCREASED MIGRATORY RESPONSES TO N-FORMYL-METHIONYL-LEUCYL-PHENYLALANINE, NEUTROPHIL-ACTIVATING FACTOR, PLATELET-ACTIVATING-FACTOR, AND PLATELET FACTOR-IV
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DOI：
10.1111/1523-1747.ep12462781
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发表时间：
1993-02-01
影响因子：
6.5
通讯作者：
KOENDERMAN, L
中科院分区：
文献类型：
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作者：
BRUIJNZEEL, PLB;KUIJPER, PHM;KOENDERMAN, L
嗜酸性粒细胞颗粒蛋白沉积在特应性皮炎皮损中。这表明嗜酸性粒细胞的活性组织浸润。为了找到嗜酸性粒细胞的组织流入的解释，嗜酸性粒细胞的迁移进行了研究，在体外通过微趋化性测定。特应性皮炎患者循环中的嗜酸性粒细胞与健康供体的嗜酸性粒细胞相比，对体外趋化刺激的反应能力发生了改变。特应性皮炎患者的嗜酸性粒细胞对N-甲酰-甲硫氨酰-亮氨酰-苯丙氨酸、嗜酸性粒细胞活化因子、血小板活化因子和血小板因子4剂量范围的迁移反应显著增加。正常人的嗜酸性粒细胞对N-甲酰-甲硫氨酰-亮氨酰-苯丙氨酸和嗜酸性粒细胞活化因子没有反应，对血小板因子4只有轻微的反应。两组对肿瘤坏死因子-α和补体因子C5 a的迁移反应相同。白细胞介素-5，嗜酸性粒细胞的选择性细胞因子，是一个强大的调节器的迁移反应，这些趋化因子在嗜酸性粒细胞从正常供体。白细胞介素-5诱导了对N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸和血小板活化因子的迁移反应，而对血小板活化因子和血小板因子4的迁移反应明显增强。相反，对补体片段C5 a的应答仅受到轻微影响。我们的研究结果表明，异位性皮炎患者的嗜酸性粒细胞对各种趋化因子的迁移反应性增加反映了嗜酸性粒细胞的体内“启动”，可能是通过循环细胞因子如白细胞介素-5。这种体内“引发”不是最佳的，因为它可以通过与白细胞介素-5的重新接触而进一步增强。
Eosinophil granular protein deposits have been demonstrated in lesional atopic dermatitis skin. This suggests active tissue infiltration of eosinophils. To find an explanation for the tissue influx of eosinophils, eosinophil migration was studied in vitro by means of a microchemotaxis assay. Eosinophils from the circulation of patients with atopic dermatitis showed an altered capacity to respond to chemotactic stimuli in vitro compared with eosinophils from healthy donors. Eosinophils from patients with atopic dermatitis had significantly increased migratory responses toward dose ranges of N-formyl-methionyl-leucyl-phenylalanine, neutrophil-activating factor, platelet-activating factor, and platelet factor 4. Eosinophils from normal individuals did not respond to N-formyl-methionyl-leucyl-phenylalanine and neutrophil-activating factor and responded only slightly to platelet factor 4. The migratory responses toward tumor necrosis factor-alpha and complement factor C5a were identical in both groups. Interleukin-5, an eosinophil-selective cytokine, is a strong modulator of the migratory responses to these chemotaxins in eosinophils from normal donors. A migratory response toward N-formyl-methionyl-leucyl-phenylalanine and neutrophil-activating factor was induced by interleukin-5, whereas the migratory response toward platelet-activating factor and platelet factor 4 was markedly potentiated. In contrast, the response to complement fragment C5a was only slightly influenced. Our findings indicate that the increased migratory responsiveness of eosinophils from patients with atopic dermatitis to various chemotaxins reflects in vivo ''priming'' of eosinophils, presumably by circulating cytokines such as interleukin-5. This in vivo ''priming'' is not optimal because it can be further potentiated by renewed contact with interleukin-5.