Biomembrane Interactions Reveal the Mechanism of Action of Surface-Immobilized Host Defense IDR-1010 Peptide
Biomembrane Interactions Reveal the Mechanism of Action of Surface-Immobilized Host Defense IDR-1010 Peptide
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DOI：
10.1016/j.chembiol.2011.12.015
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发表时间：
2012-02-24
影响因子：
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通讯作者：
Kizhakkedathu, Jayachandran N.
中科院分区：
文献类型：
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作者：
Gao, Guangzheng;Cheng, John T. J.;Kizhakkedathu, Jayachandran N.
剖析表面结合型抗菌肽和免疫调节肽的作用机理，对于优化生物医疗设备抗感染涂层的设计是至关重要的。为了解决这一问题，我们比较了宿主防御肽IDR-1010cys(1)游离形式，(2)作为可溶性聚合物结合物，以及(3)一端拴在固体载体上的生物膜与模型细菌和哺乳动物脂膜的相互作用。我们的结果表明，三种不同形式的IDR-1010cys都能与细菌和哺乳动物的脂泡相互作用，但通过诱导二级结构来监测相互作用的程度不同。表面结合多肽的相互作用增强与其良好的抗菌活性密切相关。我们的结果表明，表面醚化的IDR-1010cys的作用机制可能与游离的IDR-1010cys不同。
Dissecting the mechanism of action of surface-tethered antimicrobial and immunomodulatory peptides is critical to the design of optimized anti-infection coatings on biomedical devices. To address this, we compared the biomembrane interactions of host defense peptide IDR-1010cys (1) in free form, (2) as a soluble polymer conjugate, and (3) with one end tethered to a solid support with model bacterial and mammalian lipid membranes. Our results show that IDR-1010cys in all three distinct forms interacted with bacterial and mammalian lipid vesicles, but the extent of the interactions as monitored by the induction of secondary structure varied. The enhanced interaction of surface-tethered peptides is well correlated with their very good antimicrobial activities. Our results demonstrate that there may be a difference in the mechanism of action of surfacetethered versus free IDR-1010cys.