4 MATING-TYPE GENES CONTROL SEXUAL-DIFFERENTIATION IN THE FISSION YEAST
4 MATING-TYPE GENES CONTROL SEXUAL-DIFFERENTIATION IN THE FISSION YEAST
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DOI：
10.1002/j.1460-2075.1988.tb02973.x
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发表时间：
1988-05-01
期刊：
影响因子：
11.4
通讯作者：
BEACH, D
中科院分区：
文献类型：
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作者：
KELLY, M;BURKE, J;BEACH, D
裂殖酵母的交配型区域由三个部分组成，即mat1、mat2 - P和mat3 - M，每个部分之间相隔15kb。细胞类型由mat1上存在的交替等位基因决定，在h⁺细胞中为P，在h⁻细胞中为M。mat2 - P和mat3 - M作为信息供体，在交配型转换过程中信息会转座到mat1。我们已经确定了mat每个部分的核苷酸序列。P和M特异性区域分别为1104bp和1128bp，并由每个交配型盒共有的序列（H1；59bp和H2；135bp）界定。第三个序列存在于mat2 - P和mat3 - M，但在mat1中缺失（H3；57bp），可能参与这些盒的转录抑制。mat1 - P和mat1 - M各自编码两个基因（Pc；118个氨基酸，Pi；159个氨基酸，Mc；181个氨基酸和Mi；42个氨基酸）。在每个基因的开放阅读框中引入无义突变或移码突变表明，Pc和Mc对于交配是必需的且是充分的，分别赋予h⁺或h⁻交配型。在h⁺/h⁻二倍体中，所有四个基因对于减数分裂能力都是必需的。每个mat基因的转录受营养条件的强烈影响，并且仅在无氮培养基中观察到完全诱导。Pi基因的预测产物包含一个与同源盒序列同源的区域，这表明该基因编码一种直接调节其他基因表达的DNA结合蛋白。
The mating-type region of fission yeast consists of three components, mat1, mat2-P and mat3-M, each separated by 15 kb. Cell-type is determined by the alternate allele present at mat1, either P in an h+ or M in an h- cell. mat2-P and mat3-M serve as donors of information that is transposed to mat1 during a switch of mating type. We have determined the nucleotide sequence of each component of mat. The P and M specific regions are 1104 and 1128 bp, respectively, and bounded by sequences common to each mating-type cassette (H1; 59 bp and H2; 135 bp). A third sequence is present at mat2-P and mat3-M but absent at mat1 (H3; 57 bp), and may be involved in transcriptional repression of these cassettes, mat1-P and mat1-M each encode two genes (Pc; 118 amino acids, Pi; 159 amino acids, Mc; 181 amino acids and Mi; 42 amino acids). Introduction of opal or frame-shift mutations into the open-reading-frame of each gene revealed that Pc and Mc are necessary and sufficient for mating and confer an h+ or h- mating type respectively. All four genes are required for meiotic competence in an h+/h- diploid. The transcription of each mat gene is strongly influenced by nutritional conditions and full induction was observed only in nitrogen-free medium. The predicted product of the Pi gene contains a region of homology with the homeobox sequence, suggesting that this gene encodes a DNA binding protein that directly regulates the expression of other genes.