Components of the Arabidopsis CBF Cold-Response Pathway Are Conserved in Non-heading Chinese Cabbage
Components of the Arabidopsis CBF Cold-Response Pathway Are Conserved in Non-heading Chinese Cabbage
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DOI：
10.1007/s11105-010-0256-3
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发表时间：
2011-09-01
影响因子：
2.1
通讯作者：
Hou, Xilin
中科院分区：
文献类型：
--
作者：
Jiang, Fangling;Wang, Feng;Hou, Xilin
许多植物在暴露于低的非冻结温度时增加抗冻性，这种现象称为冷驯化。拟南芥的冷驯化涉及C-重复/脱水响应元件结合因子（CBF）表达（ICE）转录激活因子的诱导物的快速冷诱导表达，随后表达CBF;随后，CBF靶向基因增加冷冻耐受性。在这里，我们提出的CBF冷反应途径在不结球白菜（白菜ssp。Chinensis L. Makino）。我们发现，不结球白菜编码ICE 1-like基因BrICE 1，它包含一个1,491 bp的开放阅读框，编码一个具有潜在bHLH结构域的蛋白质，该结构域在低温下迅速积累，随后表达BrCBF基因，一个拟南芥CBF 3-like基因的直系同源物，然后是BrCOR 14基因，一个拟南芥CBF靶向COR 15 b基因的直系同源物。对拟南芥和甘蓝型油菜的后两个基因的比对表明，在BrCBF中存在保守的CANNTG核心元件和AP 2结构域，在BrCOR 14中存在CCG核心元件。此外，BrCBF和BrCOR 14在低温、作为盐和干旱胁迫下表达增强，但在阿坝胁迫下表达不增强，这与拟南芥相似。我们的结论是CBF冷反应途径的组成部分是高度保守的不结球白菜。
Many plants increase in freezing tolerance upon exposure to low non-freezing temperatures, a phenomenon known as cold acclimation. Cold acclimation in Arabidopsis involves rapid cold-induced expression of the inducer of C-repeat/dehydration-responsive element-binding factor (CBF) expression (ICE) transcriptional activators followed by expression of the CBF; subsequently, CBF-targeted genes that increase freezing tolerance. Here, we present evidence for a CBF cold-response pathway in non-heading Chinese cabbage (Brassica campestris ssp. chinensis L. Makino). We show that non-heading Chinese cabbage encodes ICE1-like gene BrICE1 that bracket an open reading frame of 1,491 bp encoding a protein with a potential bHLH domain, which accumulates rapidly in response to low temperature followed closely by expression of the BrCBF gene, an ortholog of the Arabidopsis CBF3-like gene, and then BrCOR14 gene, an ortholog of the Arabidopsis CBF-targeted COR15b gene. An alignment of the later two genes from Arabidopsis, Brassica napus revealed the presence of conserved CANNTG core element and AP2 domain in BrCBF and a CCG core element in BrCOR14. In addition, BrCBF and BrCOR14 showed increased expression induced by low temperature as well as salt and drought, but not by ABA stress which are similar to those of Arabidopsis. We conclude that components of the CBF cold-response pathway are highly conserved in non-heading Chinese cabbage.