Effect of Low-Level Laser Therapy (660 nm) on Angiogenesis in Wound Healing: A Immunohistochemical Study in a Rodent Model
Effect of Low-Level Laser Therapy (660 nm) on Angiogenesis in Wound Healing: A Immunohistochemical Study in a Rodent Model
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DOI：
10.1590/0103-6440201301867
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发表时间：
2013-07-01
影响因子：
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通讯作者：
Reis, Silvia Regina de Almeida
中科院分区：
文献类型：
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作者：
Colombo, Fabio;Neto, Alberto de Aguiar Pires Valenca;Reis, Silvia Regina de Almeida
本研究的目的是评价用660 nm激光治疗的啮齿动物模型背部皮肤伤口的血管生成。新血管的形成是一个多步骤的过程，涉及血管发芽，内皮细胞迁移，增殖和管形成。虽然几项体内研究表明激光光疗影响组织修复，但对血管生成机制的全面了解尚不清楚。使用24只体重在200和250 g之间的年轻成年雄性Wistar大鼠。在全身麻醉下，在每只动物的背部上创建一个切除伤口，并将它们随机分为两组：一组为对照组，另一组为激光治疗组（660 nm，16 mW，10 J/cm 2）。根据动物死亡时间（2、4和6天）将每组再分为3个亚组。手术后立即开始激光照射，并在实验期间每隔一天重复一次，用天狼星红标记，对胶原蛋白特异性，并用抗TGF-β和抗von Willebrand因子免疫标记。通过光学显微镜对标记的切片进行组织学分析，计算每只动物的平均伤口面积，并通过ANOVA和Tukey检验（α =0.05）进行分析。尽管在某些死亡时期，照射动物的胶原蛋白表达和血管数量高于对照动物，但在任何时间均未发现与TGF-β表达相关的显着差异（p>0.05）。结论：激光治疗（660 nm）有助于增加血管生成。
The aim of the present investigation was to evaluate the angiogenesis on dorsal cutaneous wounds in a rodent model treated with lambda660 nm laser light. New vessel formation is a multistep process involving vessel sprouting, endothelial cell migration, proliferation and tube formation. Although several in vivo studies have shown that laser phototherapy influences tissue repair, a fully understanding of angiogenesis mechanisms are not yet known. Twenty-four young adult male Wistar rats weighing between 200 and 250 g were used. Under general anesthesia, one excisional wound was created on the dorsum of each animal and they were randomly distributed into two groups: one control and one treated with laser (lambda660 nm, 16 mW, 10 J/cm2). Each group was subdivided into three subgroups according to the animal death timing (2, 4 and 6 days). Laser irradiation started immediately after surgery and was repeated every other day during the experiment and marked with Sirius Red, specific for collagen, and immunomarked with anti-TGF-beta and anti-von Willebrand factor. Marked sections underwent histological analysis by light microscopy and the mean area of the wound of each animal was calculated and analyzed by ANOVA and Tukey's test (alpha=0.05). Although at some death periods, collagen expression and number of blood vessels on irradiated animals were higher than in the control ones, no significant differences were found at any time in relation to TGF-beta expression (p>0.05). It was concluded that laser treatment (lambda660 nm) contributed to increase angiogenesis.