Pathogenesis and phylogenetic analyses of canine distemper virus strain ZJ7 isolate from domestic dogs in China.
Pathogenesis and phylogenetic analyses of canine distemper virus strain ZJ7 isolate from domestic dogs in China.
复制标题
DOI：
10.1186/1743-422x-8-520
复制
发表时间：
2011-11-16
期刊：
影响因子：
4.8
通讯作者：
Wu H
中科院分区：
文献类型：
--
作者：
Tan B;Wen YJ;Wang FX;Zhang SQ;Wang XD;Hu JX;Shi XC;Yang BC;Chen LZ;Cheng SP;Wu H
用MDCK细胞从疑似犬瘟热病毒（CDV）感染犬的肺组织中分离到一株新的CDV，命名为ZJ 7。ZJ 7分离物在MDCK细胞中传代6代后可引起合胞体的致细胞病变作用。为了解ZJ 7株的致病性，用ZJ 7株病毒悬液鼻内接种3只犬瘟热血清阴性犬。所有感染的狗在21 dpi时出现严重的血性腹泻、结膜炎、眼分泌物、鼻涕和咳嗽、发热和体重减轻的临床体征，而感染DMEM的模拟组是正常的。结果表明，用MDCK细胞分离的CDV-ZJ 7株具有强毒力，经MDCK细胞分离后，ZJ 7株的核苷酸和氨基酸序列与从新鲜组织中分离的原始病毒相比无明显变化。ZJ 7分离株的核壳（N）、磷蛋白（P）和受体结合血凝素（H）基因的分子和系统发育分析清楚地表明，它属于CDV毒株的Asia 1组簇，这是中国的主要基因型。
A new isolate of canine distemper virus (CDV), named ZJ7, was isolated from lung tissues of a dog suspected with CDV infection using MDCK cells. The ZJ7 isolate induced cytopathogenic effects of syncytia in MDCK cell after six passages. In order to evaluate pathogenesis of ZJ7 strain, three CDV sero-negative dogs were intranasally inoculated with its virus suspension. All infected dogs developed clinical signs of severe bloody diarrhea, conjunctivitis, ocular discharge, nasal discharge and coughing, fever and weight loss at 21 dpi, whereas the mock group infected with DMEM were normal. The results demonstrated that CDV-ZJ7 strain isolated by MDCK cell was virulent, and the nucleotide and amino acid sequences of strain ZJ7 had no change after isolation by MDCK cell when compared with the original virus from the fresh tissues. Molecular and phylogenetic analyses for the nucleocapsid (N), phosphoprotein (P) and receptor binding haemagglutinin (H) gene of the ZJ7 isolate clearly showed it is joins to the Asia 1 group cluster of CDV strains, the predominant genotype in China.