DNA barcodes to identify species and explore diversity in the Adelgidae (Insecta: Hemiptera: Aphidoidea)
DNA barcodes to identify species and explore diversity in the Adelgidae (Insecta: Hemiptera: Aphidoidea)
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DOI：
10.1111/j.1755-0998.2009.02644.x
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发表时间：
2009-05-01
影响因子：
7.7
通讯作者：
Montgomery, M. E.
中科院分区：
文献类型：
--
作者：
Foottit, R. G.;Maw, H. E. L.;Montgomery, M. E.
球蚜科是一种相对较小的隐蔽性昆虫，具有复杂的生活史和孤雌生殖。由于这些特征，该群体的分类是有问题的。在这里，我们测试的有效性标准658 bp的条形码片段从5 '端的线粒体细胞色素c氧化酶1基因（COI）在区分17种球蚜科，在关联的生命周期阶段，并在选定的物种的地理变异模式进行评估。通过DNA条形码可以很好地区分Adelgidae的物种，从而能够识别不同宿主和不同生命周期时期的不同形态形式，未成熟阶段和个体。DNA条形码揭示了分类群内的隐藏多样性，在其他情况下，以前由生命周期特征分开的物种对缺乏序列差异，这表明需要进一步的分类学分析。
The Adelgidae are relatively small, cryptic insects, exhibiting complex life cycles with parthenogenetic reproduction. Due to these characteristics, the taxonomy of the group is problematic. Here, we test the effectiveness of the standard 658-bp barcode fragment from the 5'-end of the mitochondrial cytochrome c oxidase 1 gene (COI) in differentiating among 17 species of Adelgidae, in associating life-cycle stages, and in assessing patterns of geographical variation in selected species. Species of Adelgidae are well-differentiated by DNA barcodes, enabling the identification of different morphological forms, immature stages and individuals on different hosts and at different periods of the life cycle. DNA barcodes have uncovered cryptic diversity within taxa and, in other cases, a lack of sequence divergence in species pairs previously separated by life-cycle characteristics, indicating a need for further taxonomic analysis.