Quantitative and Qualitative Estimation of Bacteria Contaminating Human Hairs
Quantitative and Qualitative Estimation of Bacteria Contaminating Human Hairs
复制标题
污染人类头发的细菌的定量和定性估计
DOI：
10.4167/jbv.2010.40.1.11
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发表时间：
2010
影响因子：
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通讯作者：
D. Park
中科院分区：
文献类型：
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作者：
Aram Yun;E. Yang;Young Mi Lee;Seon;H. Seo;D. Park
人类的头发很容易被微生物污染。这项研究是为了测量哪些细菌种类和多少微生物污染了特定部位的人类头发。在实验室内角和窗旁的6个位置放置未加工的人发7天。用最可能数法测定染染头发的活菌细胞数，最大可达10？/g头发，内角最少10³/g头发，最多10？/g毛，窗边毛最少10³/g。采用4′，6-二氨基-2-苯基吲哚（4′，6-二氨基-2-苯基吲哚，DAPI）染色，在荧光显微镜下观察染染人毛发的细菌细胞。基于16S-rDNA可变区多样性，采用热梯度凝胶电泳（TGGE）技术对染发细菌群落进行分析。总共从12组头发样本中检测到大约20种细菌。本研究将荧光染色、TGGE和mpn这三种常用的实验方法相结合，建立了一种新的观察和估计头发细菌污染的方法。
Human hairs have been known to be easily contaminated with microorganisms. This study was performed in order to measure what bacterial species and how much microorganisms contaminate human hairs in specific place. Virgin human hairs were left at 6 positions in inside corner and beside window in a laboratory for 7 days. The number of viable bacterial cells, which were determined by most probable number method, contaminating the human hairs was measured at a maximum of 10?/g hair and a minimum of 10³/g hair in inside corner and maximum of 10?/g hair and a minimum of 10³/g hair beside window. The bacterial cells-contaminating human hairs were observed via fluorescence light microscopy after 4',6-diamino-2-phenylindole (DAPI) staining. The bacterial community contaminating human hairs was analyzed via the thermal gradient gel electrophoresis (TGGE) technique, based on the diversity of the 16S-rDNA variable region. In total, approximately 20 bacterial species were detected from 12 groups of hair samples. In this study, general experimental methods-fluorescence staining, TGGE and MPN-were combined to develop new method for observation and estimation of bacteria contaminating human hairs.
H.Miyata、T.Nakao、O.Aozasa、S.Ohta：“评估人体对 PCDD 的暴露。使用头发作为人体污染指标样品的 PCDFS 和 Co-PCB I：开发人类头发的分析方法和暴露评估”
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