Transcriptional profiling of canola developing embryo and identification of the important roles of BnDof5.6 in embryo development and fatty acids synthesis
Transcriptional profiling of canola developing embryo and identification of the important roles of BnDof5.6 in embryo development and fatty acids synthesis
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双低油菜籽发育胚胎的转录谱及BnDof5.6在胚胎发育和脂肪酸合成中重要作用的鉴定
DOI：
10.1093/pcp/pcv074
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发表时间：
2015-08-01
影响因子：
4.9
通讯作者：
Yuan, Yujin
中科院分区：
文献类型：
--
作者：
Deng, Wei;Yan, Fang;Yuan, Yujin
油菜籽是全球重要的植物油作物，了解种胚发育过程中脂肪酸生物合成的分子机制是一个重要的研究目标。在这里，我们报告了利用RNA测序(RNA-Seq)方法对油菜胚胎发育过程中的转录谱进行分析。RNA-Seq分析产生58,579,451个序列读数，与32,243个基因比对。研究发现，共有55个差异表达基因(DEG)编码28个酶，在碳流到储藏标签脂肪酸中起作用。大多数编码上述酶的DEGS具有相似的表达模式，表明DEGS协同参与了碳流向脂肪酸的过程。此外，在RNA-Seq数据库中还发现了与生长素、赤霉素、脱落酸、细胞分裂素和水杨酸的信号转导、运输和代谢过程相关的41个序列，这表明植物激素在控制胚胎发育和脂肪酸合成中发挥着重要作用。在油菜胚胎发育过程中发现了122个转录因子家族成员的编码序列。此外，在RNA-Seq数据库中发现的锌指转录因子基因BnDOF5.6在油菜胚胎发育过程中下调。转基因植株的胚胎尺寸变小，脂肪酸含量降低，种子脂肪酸组成发生变化。BnDof5.6的下调也改变了与脂肪酸合成和去饱和有关的基因的表达水平。我们的结果表明，BnDof5.6是油菜胚胎发育和脂肪酸合成所必需的。总之，这项研究提供了关于胚胎发育过程中基因全球表达模式的新信息，并将扩大我们对碳流到脂肪酸和油菜胚胎发育的复杂分子机制的理解。
Canola is an important vegetable oil crop globally, and the understanding of the molecular mechanism underlying fatty acids biosynthesis during seed embryo development is an important research goal. Here we report the transcriptional profiling analysis of developing canola embryos using RNA-sequencing (RNA-Seq) method. RNA-Seq analysis generated 58,579,451 sequence reads aligned with 32,243 genes. It was found that a total of 55 differential expression genes (DEGs) encoding 28 enzymes function in carbon flow to fatty acids of storage TAG. Most of the DEGs encoding above enzymes showed similar expression pattern, indicating the DEGs are cooperatively involved in carbon flow into fatty acids. In addition, 41 DEGs associated with signal transductions, transport and metabolic processing of auxin, gibberellin, abscisic acid, cytokinin and salicylic acids were found in the RNA-Seq database, which indicates the important roles of the phytohormones in controlling embryo development and fatty acids synthesis. 122 DEGs encoding transcriptional factor family members were found in developing canola embryos. Furthermore, BnDOF5.6, a zinc finger transcriptional factor gene, found in RNA-Seq database was down-regulated in developing canola embryos. The transgenic plants displayed reduced embryo sizes, decreased fatty acids contents and altered seed fatty acids composition in canola. Down-regulated of BnDof5.6 also changed the expression levels of genes involved in fatty acids synthesis and desaturation. Our results indicate that BnDof5.6 is required for embryo development and fatty acids synthesis in canola. Overall this study presents new information on the global expression patterns of genes during embryo development and will expand our understanding of the complex molecular mechanism of carbon flow into fatty acids and embryo development in canola.