GABA in locus coeruleus regulates spontaneous rapid eye movement sleep by acting on GABA(A) receptors in freely moving rats
GABA in locus coeruleus regulates spontaneous rapid eye movement sleep by acting on GABA(A) receptors in freely moving rats
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DOI：
10.1016/s0304-3940(97)13410-3
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发表时间：
1997-02-21
影响因子：
2.5
通讯作者：
Mallick, BN
中科院分区：
文献类型：
--
作者：
Kaur, S;Saxena, RN;Mallick, BN
已知蓝斑中的胺能神经元在快速眼动睡眠期间停止放电。由于蓝斑的电刺激减少，而卡巴胆碱刺激增加了快速眼动睡眠，并且蓝斑中存在γ-氨基丁酸（GABA）神经元和末梢，因此我们假设 GABA 可能参与快速眼动睡眠期间蓝斑神经元放电的停止。在手术麻醉下，将带有针对蓝斑的双侧引导插管的雄性 Wistar 大鼠（250-300 g）准备用于慢性睡眠-觉醒记录。在正常情况下、在250nl盐水后和在双侧蓝斑注射印防己毒素（250ng/250nl）后记录脑电图（EEG）、眼电图（EGG）、肌电图（EMG）。结果表明，快速动眼睡眠每次发作的平均持续时间显着缩短，但每小时的生成频率并未受到显着影响。这项研究表明蓝斑中的 GABA 参与快速动眼睡眠的强直调节，并且该作用是通过 GABAA 受体介导的。 (C) 1997 爱思唯尔科学爱尔兰有限公司
The aminergic neurons in the locus coeruleus are known to cease firing during rapid eye movement sleep. Since electrical stimulation of locus coeruleus reduced, while carbachol stimulation increased rapid eye movement sleep and gamma-aminobutyric acid (GABA) neurons as well as terminals are present in the locus coeruleus, we hypothesized that GABA may be involved for cessation of locus coeruleus neuronal firing during rapid eye movement sleep. Under surgical anaesthesia male Wistar rats (250-300 g) with bilateral guide cannulae targeting locus coeruleus were prepared for chronic sleep-wakefulness recording. Electroencephalogram (EEG), electrooculogram (EGG), electromyogram (EMG) were recorded in normal, after 250 nl saline and after picrotoxin (250 ng in 250 nl) injection bilaterally into the locus coeruleus. The results showed that mean duration per episode of rapid eye movement sleep was significantly reduced, although its frequency of generation/h was not significantly affected. This study suggests that GABA in locus coeruleus is involved in tonic regulation of rapid eye movement sleep and the action is mediated through GABAA receptor. (C) 1997 Elsevier Science Ireland Ltd.