Coral snake antivenom produced in chickens (Gallus domesticus).
Coral snake antivenom produced in chickens (Gallus domesticus).
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DOI：
10.1590/s0036-46652014000100009
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发表时间：
2014-01
影响因子：
1.9
通讯作者：
Rodriguez-Acosta FA
中科院分区：
文献类型：
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作者：
Aguilar I;Sánchez EE;Girón ME;Estrella A;Guerrero B;Rodriguez-Acosta FA
从大型哺乳动物的血液中生产抗蛇毒产量低且困难，因此，用于治疗的抗蛇毒免疫球蛋白通常作为多价血清获得。本研究标准化了一种从免疫母鸡蛋黄中制备含珊瑚蛇毒多克隆抗体的纯化免疫球蛋白IgY抗蛇毒血清的简易方法。我们采用了一种从蛋黄中纯化抗体的报道过程，并获得了90%的抗体纯度。定制的技术包括通过冻融顺序从蒸馏水稀释的蛋黄中去除脂质。特异性免疫球蛋白在免疫后的180天内仍存在于蛋黄中。因此，通过少量的毒液，在充分纯化的状态下发现了大量的免疫球蛋白（获得的物质含有约90%的纯IgY）。采用双免疫扩散试验检测免疫球蛋白的抗原结合。用血清保护试验（中位有效剂量= ED50） （ED50= 477 mg/kg）估计蛋黄中抗体滴度。鉴于养殖母鸡在经济上可行，鸡蛋采集无创，IgY抗体的纯化快速简便，鸡免疫是生产多克隆抗体的良好选择。据我们所知，这是第一次在鸟类中制备珊瑚蛇抗蛇毒血清。
The production of anti-snake venom from large mammal's blood has been found to be low-yielding and arduous, consequently, antivenom immunoglobulins for treatment are achieved regularly as polyvalent serum. We have standardized an undemanding technique for making purified immunoglobulin IgY antivenom consisting of polyclonal antibodies against coral snake venom in the egg yolk of immunized hens. We have adapted a reported process of antibody purification from egg yolks, and achieved 90% antibody purity. The customized technique consisted of the removal of lipids from distilled water-diluted egg yolks by a freeze–thaw sequence. The specific immunoglobulins were present in the egg yolk for up to 180 days postimmunization. Therefore, by means of small venom quantities, a significant amount of immunoglobulins were found in an adequately purified state (The obtained material contained about 90% pure IgY). The antigen binding of the immunoglobulins was detected by a double immunodiffusion test. Titers of antibodies in the yolk were estimated with a serum protection assay (Median effective dose = ED50) (ED50= 477 mg/kg). Given that breeding hens is economically feasible, egg gathering is noninvasive and the purification of IgY antibodies is quick and easy, chicken immunization is an excellent alternative for the production of polyclonal antibodies. To the best of our knowledge, this is the first coral snake antivenom prepared in birds.