Expression of somatostatin in the adult and developing mouse kidney.
Expression of somatostatin in the adult and developing mouse kidney.
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生长抑素在成年和发育中小鼠肾脏中的表达。
DOI：
10.1111/j.1523-1755.2004.00953.x
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Grady,Sandy
中科院分区：
文献类型：
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作者：
Bates,CarltonM;Kegg,Heather;Grady,Sandy
生长抑素（somatostatin，生长激素释放抑制因子）（SRIF）具有较强的抗增殖和抗分泌作用。在成人肾脏中，生长抑素改变肾血流量、离子转运和水渗透性。虽然一些证据表明SRIF可能由成年肾小管细胞产生，但产生SRIF的特定小管尚不清楚。生长抑素也已在多种胚胎组织中检测到，尽管尚未在发育中的肾脏中描述。我们的目的是确定SRIF在成年和胚胎小鼠kidney.MethodsWe的表达模式进行逆转录-聚合酶链反应（RT-PCR）和免疫荧光SRIF在发育和成年小鼠肾组织。我们本地化SRIF双或系列标记免疫荧光与特定markers.ResultsSomatostatin mRNA存在于整个胚胎发育和成年期的肾脏。从胚胎第12.5天开始，SRIF在后肾间充质细胞的界面和输尿管芽干的基底外侧表面强烈表达。从E16.5开始，界面处的染色仅限于外周输尿管芽干和新分裂的输尿管芽壶腹的裂隙。在较老的胚胎中，SRIF也出现在髓质小管中，这些小管似乎正在成熟Henle的细降支。在成人肾脏，SRIF蛋白只定位于髓薄降支的Henle loop.ConclusionIn胚胎肾脏，SRIF表达首先在接口的后肾间充质和基底外侧输尿管芽，后来在成熟薄降支的Henle。在成人肾脏中，细降支中的表达持续存在。
Expression of somatostatin in the adult and developing mouse kidney.BackgroundSomatostatin (somatotropin release inhibiting factor) (SRIF) has potent antiproliferative and antisecretory actions. In the adult kidney, somatostatin alters renal blood flow, ion transport, and water permeability. While some evidence suggests that SRIF may be produced by adult kidney tubular cells, the specific tubules generating SRIF are unknown. Somatostatin has also been detected in a variety of embryonic tissues, although it has not been described in the developing kidney. Our objective was to determine the expression pattern of SRIF in both the adult and embryonic mouse kidney.MethodsWe performed reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence for SRIF in developing and adult mouse kidney tissues. We localized SRIF by dual or serial labeling immunofluorescence with specific markers.ResultsSomatostatin mRNA was present in kidneys throughout embryogenesis and into adulthood. Starting at embryonic day (E) 12.5, SRIF was strongly expressed at the interface of the metanephric mesenchymal cells and the basolateral surfaces of ureteric bud trunks. Starting at E16.5, the staining at the interface was confined to the peripheral ureteric bud trunks and the clefts of newly dividing ureteric bud ampullae. In older embryos, SRIF also appeared in medullary tubules that appeared to be maturing thin descending limbs of Henle. In the adult kidney, SRIF proteins localized exclusively to medullary thin descending limbs of the Henle loop.ConclusionIn embryonic kidneys, SRIF is expressed first at the interface of the metanephric mesenchyme and basolateral ureteric bud and later in maturing thin descending limbs of Henle. Expression in the thin descending limb persists in the adult kidney.