ROLE OF CYTOCHROME-B5 IN THE CYTOCHROME-P-450-MEDIATED C21-STEROID 17,20-LYASE REACTION
ROLE OF CYTOCHROME-B5 IN THE CYTOCHROME-P-450-MEDIATED C21-STEROID 17,20-LYASE REACTION
复制标题
DOI：
10.1016/0006-291x(82)91877-0
复制
发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
FUJIMURA, Y
中科院分区：
文献类型：
--
作者：
KATAGIRI, M;SUHARA, K;FUJIMURA, Y
从猪睾丸微粒体中广泛纯化了细胞色素P-450（P-450 sccII）及其还原酶NADPH-细胞色素还原酶[EC 1.6.2.4]，其与孕酮转化为4-雄甾烯-3，17-二酮有关。在NADPH、NADH和O2存在下，通过混合P-450 sccII、其还原酶、猪肝细胞色素b5和细胞色素b5-还原酶[EC 1.6.2.2]以及磷脂，可以恢复较高的裂解酶活性（每摩尔P-450每分钟形成15摩尔产物的转换数）。省略细胞色素b5或NADH导致裂解酶活性的显著损失，表明细胞色素b5实际参与了睾丸中P-450介导的类固醇生成系统。
The cytochrome P-450 (P-450sccII) and its reductase, NADPH-cytochrome reductase [EC 1.6.2.4], associated with conversion of progesterone to 4-androstene-3,17-dione, were extensively purified from pig testis microsomes. Higher lyase activity (turnover number of 15 mol of the product formed per min per mol of P-450) could be restored by mixing the P-450sccII, its reductase, pig liver cytochrome b5 and cytochrome b5-reductase [EC 1.6.2.2], and phospholipid in the presence of NADPH, NADH and O2. Omission of cytochrome b5 or NADH resulted in a significant loss of the lyase activity, indicating actual participation of cytochrome b5 in this P-450-mediated steroidogenic system in the testis.