Rab32, a novel Rab small GTPase from orange-spotted grouper, Epinephelus coioides involved in SGIV infection
Rab32, a novel Rab small GTPase from orange-spotted grouper, Epinephelus coioides involved in SGIV infection
复制标题
DOI：
10.1016/j.fsi.2023.109229
复制
发表时间：
2023-11-20
影响因子：
4.7
通讯作者：
Wang，Shaowen
中科院分区：
文献类型：
--
作者：
Wang，Liqun;Zhang，Xinyue;Wang，Shaowen
Rab32是Rab GT3家族的成员，其参与膜运输和免疫应答，这对于控制病原体感染至关重要。然而，Rab32在病毒感染中的作用还不清楚。本研究重点研究Rab 32对斜带石斑鱼病毒感染和宿主免疫的调节作用。EcRab32编码213个氨基酸，与鱼类和哺乳动物Rab32蛋白序列高度同源。在健康橙斑石斑鱼体内，EcRab32 mRNA在所有检测组织中都有表达，其中头肾、肝脏和鳃中表达量较多。SGIV感染后，EcRab32的表达在体外显著上调，提示其在病毒感染中的潜在作用。观察到EcRab32以点状和囊泡样结构分布在细胞质中。发现EcRab32过表达显著抑制SGIV感染，而EcRab32的中断显著促进SGIV感染。此外，使用单颗粒成像分析，我们发现EcRab32过表达显著降低SGIV颗粒的附着和内化。此外，结果表明，EcRab32在干扰素免疫和炎症反应中发挥积极的调节作用。综上所述，这些发现表明EcRab32通过调节宿主免疫应答来影响SGIV感染，提供了Rab32和先天免疫之间相互作用的整体理解。
Rab32 is a member of the Rab GTPase family that is involved in membrane trafficking and immune response, which are crucial for controlling pathogen infection. However, the role of Rab32 in virus infection is not well understood. In this study, we focused on the regulation of Rab32 on virus infection and the host immunity in orange-spotted grouper, Epinephelus coioides. EcRab32 encoded a 213-amino acid polypeptide, which shared a high sequence identity with other Rab32 proteins from fishes to mammals. In healthy orange-spotted grouper, the mRNA of EcRab32 was expressed in all the detected tissues, with the more expression levels in the head kidney, liver and gill. Upon SGIV infection, the expression of EcRab32 was significantly up-regulatedin vitro, indicating its potential role in viral infection. EcRab32 was observed to be distributed in the cytoplasm as punctate and vesicle-like structures. EcRab32 overexpression was found to notably inhibit SGIV infection, while the interruption of EcRab32 significantly promoted SGIV infection. In addition, using single particle imaging analysis, we found that EcRab32 overexpression prominently reduced the attachment and internalization of SGIV particles. Furthermore, the results demonstrated that EcRab32 played a positive role in regulating the interferon immune and inflammatory responses. Taken together, these findings indicated that EcRab32 influenced SGIV infection by regulating the host immune response, providing an overall understanding of the interplay between the Rab32 and innate immunity.